Home
  >  
Section 69
  >  
Chapter 68,568

Molecular cloning and nucleotide sequence of cDNAs encoding the precursors of rat long chain acyl-coenzyme A, short chain acyl-coenzyme A, and isovaleryl-coenzyme a dehydrogenases. Sequence homology of four enzymes of the acyl-CoA dehydrogenase family

Matsubara, Y.; Indo, Y.; Naito, E.; Ozasa, H.; Glassberg, R.; Vockley, J.; Ikeda, Y.; Kraus, J.; Tanaka, K.

Journal of Biological Chemistry 264(27): 16321-16331

1989

ISSN/ISBN: 0021-9258
PMID: 2777793
Accession: 068567542
Download citation:  
Text
  |  
BibTeX
  |  
RIS
CDNAs encoding the entire coding regions of the precursors (p) of rat long chain acyl-CoA (LCAD), short chain acyl-CoA (SCAD) and isovaleryl-CoA dehydrogenase (IVD) have been cloned and sequenced. Three cDNAs for rat liver LCAD together cover a 1440-base pair region. These cDNAs encode the entire 430-amino acid sequence of pLCAD, including the 30-amino acid leader peptide and the 400-amino acid mature LCAD. A single 1773 base pair cDNA for rat SCAD covers the entire coding region (414 amino acids), including the 26-amino acid leader peptide and the 388-amino acid mature peptide. Four identified IVD cDNAs, when combined, encompass a 2104 base region, and encode 424 amino acids including a 30-amino acid leader peptide and the 394-amino acid mature peptide. The identities of all cDNA clones have been confirmed by matching the amino acid sequences predicted from the respective cDNAs to the amino-terminal and tryptic peptide sequences derived from the corresponding purified rat enzyme. Comparison of the sequences of four rat acyl-CoA dehydrogenases, including LCAD, MCAD, SCAD, and IVD, and two of their human counterparts (MCAD and SCAD) reveals a high degree of homology (57 invariant and 92 near invariant residues: 30.6-35.4% of identical residues in pairwise comparisons), suggesting that these enzymes belong to a gene family and have evolved from a common ancestral gene.

PDF emailed within 1 workday: $29.90




Related References

Naito, E.; Ozasa, H.; Ikeda, Y.; Tanaka, K. 1989: Molecular cloning and nucleotide sequence of complementary DNAs encoding human short chain acyl-coenzyme A dehydrogenase and the study of the molecular basis of human short chain acyl-coenzyme A dehydrogenase deficiency Journal of Clinical Investigation 83(5): 1605-1613
Naito, E.; Ozasa, H.; Ikeda, Y.; Tanaka, K. 1990: Molecular cloning and nucleotide sequence of complementary dna encoding human short chain acyl coenzyme a dehydrogenase and study of the molecular basis of human short chain acyl coenzyme a dehydrogenase deficiency Tanaka, K And P M Coates (Ed ) Progress in Clinical And Biological Research, Vol 321 Fatty Acid Oxidation: Clinical, Biochemical, And Molecular Aspects; International Symposium, Philadelphia, Pennsylvania, Usa, November 6-9, 1988 Xxii+728p Alan R Liss, Inc : New York, New York, Usa Illus 625-632
Tanaka, K.; Matsubara, Y.; Indo, Y.; Naito, E.; Kraus, J.; Ozasa, H. 1990: The acyl coenzyme a dehydrogenase family homology and divergence of primary sequence of four acyl coenzyme a dehydrogenases and consideration of their functional significance Tanaka, K And P M Coates (Ed ) Progress in Clinical And Biological Research, Vol 321 Fatty Acid Oxidation: Clinical, Biochemical, And Molecular Aspects; International Symposium, Philadelphia, Pennsylvania, Usa, November 6-9, 1988 Xxii+728p Alan R Liss, Inc : New York, New York, Usa Illus 577-598
Ozasa, H.; Ikeda, I.; Finocchiaro, G.; Matsubara, Y.; Tanaka, K. 1987: Molecular cloning and nucleotide sequence of complementary dna encoding rat liver short chain acyl coenzyme a dehydrogenase American Journal of Human Genetics 41(3 Suppl): A232
Indo, Y.; Ozasa, H.; Ikeda, Y.; Tanaka, K. 1990: Molecular cloning and nucleotide sequence of complementary dna encoding the rat long chain acyl coenzyme a dehydrogenase precursor Tanaka, K And P M Coates (Ed ) Progress in Clinical And Biological Research, Vol 321 Fatty Acid Oxidation: Clinical, Biochemical, And Molecular Aspects; International Symposium, Philadelphia, Pennsylvania, Usa, November 6-9, 1988 Xxii+728p Alan R Liss, Inc : New York, New York, Usa Illus 633-636
Indo, Y.; Ozasa, H.; Ikeda, Y.; Tanaka, K. 1988: Molecular cloning and nucleotide sequence of complementary dna encoding rat liver long chain acyl coenzyme a dehydrogenase lcad American Journal of Human Genetics 43(3 Suppl): A188
Finocchiaro, G.; Tanaka, K. 1986: Purification and characterization of short chain and medium chain acyl coenzyme a dehydrogenases and of isovaleryl coenzyme a dehydrogenase from human liver Federation Proceedings 45(6): 1889
Naito, E.; Ozasa, H.; Ikeda, Y.; Tanaka, K. 1988: Molecular cloning and nucleotide sequence of complementary dna encoding human short chain acyl coenzyme a dehydrogenase scad and a study of its genetic deficiency American Journal of Human Genetics 43(3 Suppl): A197
Ikeda, Y.; Okamura-Ikeda, K.; Tanaka, K. 1985: Spectroscopic analysis of the interaction of rat liver short-chain, medium-chain, and long-chain acyl coenzyme a dehydrogenases with acyl coenzyme a substrates Biochemistry 24(25): 7192-7199
Matsubara, Y.; Kraus, J.P.; Ozasa, H.; Finocchiaro, G.; Glassberg, R.; Ikeda, Y.; Mole, J.; Rosenberg, L.E.; Tanaka, K. 1987: Molecular cloning and nucleotide sequence of complementary dna encoding rat medium chain acyl coenzyme a dehydrogenase mcad Pediatric Research 21(4 Part 2): 292A
Matsubara, Y.; Kraus, J.P.; Ozasa, H.; Glassberg, R.; Finocchiaro, G.; Ikeda, Y.; Mole, J.; Rosenberg, L.E.; Tanaka, K. 1987: Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme a dehydrogenase Journal of Biological Chemistry 262(21): 10104-10108
Matsubara, Y.; Kraus, J.P.; Ozasa, H.; Glassberg, R.; Finocchiaro, G.; Ikeda, Y.; Mole, J.; Rosenberg, L.E.; Tanaka, K. 1987: Molecular cloning and nucleotide sequence of complementary dna encoding the entire precursor of rat liver medium chain acyl coenzyme a dehydrogenase Journal of Biological Chemistry 262(21): 10104-10108
Naito, E.; Ozasa, H.; Ikeda, Y.; Tanaka, K. 1990: Molecular cloning and nucleotide sequence of cDNAs encoding human short chain acyl-CoA dehydrogenase and study of the molecular basis of human short chain acyl-CoA dehydrogenase deficiency Progress in Clinical and Biological Research 321: 625-632
De Bellis, L.; Gonzali, S.; Alpi, A.; Hayashi, H.; Hayashi, M.; Nishimura, M. 2000: Purification and characterization of a novel pumpkin short-chain acyl-coenzyme A oxidase with structural similarity to acyl-coenzyme A dehydrogenases Plant Physiology 123(1): 327-334
D.D.nato S.; Gellera, C. 1990: Short chain and medium chain acyl coenzyme a dehydrogenase are lowered in riboflavin responsive lipid myopathies with multiple acyl coenzyme a dehydrogenase deficiency Tanaka, K And P M Coates (Ed ) Progress in Clinical And Biological Research, Vol 321 Fatty Acid Oxidation: Clinical, Biochemical, And Molecular Aspects; International Symposium, Philadelphia, Pennsylvania, Usa, November 6-9, 1988 Xxii+728p Alan R Liss, Inc : New York, New York, Usa Illus 325-332
Naito, E.; Indo, Y.; Tanaka, K. 1990: Identification of two variant short chain acyl-coenzyme a dehydrogenase alleles, each containing a different point mutation in a patient with short chain acyl-coenzyme a dehydrogenase deficiency Journal of Clinical Investigation 85(5): 1575-1582
Gregersen, N.; Kolvraa, S. 1984: Medium chain acyl coenzyme a dehydrogenase deficiency apparent michaelis constant and maximal velocity values for fibroblast acyl coenzyme a dehydrogenase towards octanoyl coenzyme a in patient and control cell lines Journal of Inherited Metabolic Disease 7(Suppl 2): 105-106
Indo, Y.; Yang-Feng, T.; Glassberg, R.; Tanaka, K. 1992: Molecular cloning and nucleotide sequence of cDNAs encoding human long chain acyl-CoA dehydrogenase and assignment of its gene to chromosome 2 Progress in Clinical and Biological Research 375: 161-167
Ikeda, Y.; Keese, S.M.; Coates, P.M.; Tanaka, K. 1985: Molecular study of mutant medium chain acyl coenzyme a dehydrogenase in cultured fibroblasts from patients with medium chain acyl coenzyme a dehydrogenase deficiency Pediatric Research 19(4 Part 2): 249A
Hazekawa, I.; Nishina, Y.; Sato, K.; Shichiri, M.; Shiga, K. 1995: Substrate activating mechanism of short-chain acyl-CoA, medium-chain acyl-CoA, long-chain acyl-CoA, and isovaleryl-CoA dehydrogenases from bovine liver: a resonance Raman study on the 3-ketoacyl-CoA complexes Journal of Biochemistry 118(5): 900-910