+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ultrastructural localization of insulin-like growth factor-2 (IGF-2) to the secretory granules of insulin cells: a study in normal and diabetic (GK) rats



Ultrastructural localization of insulin-like growth factor-2 (IGF-2) to the secretory granules of insulin cells: a study in normal and diabetic (GK) rats



Ultrastructural Pathology 21(5): 457-466



By using biochemical and light-microscopical techniques, insulin-like growth factor-2 (IGF-2) has recently been found in adult pancreas, co-localized immunohistochemically with insulin in the islet B-cells. The purpose of this study was to trace IGF-2 immunoreactivity (IR) at the ultrastructural level in normal and diabetic Goto-Kakizaki (GK) rats. Using a pre-embedding technique and immuno-gold-silver staining, IGF-2 antibody binding was localized exclusively to the halo of a subset of secretory beta-granules in normal rats. Insulin IR occurred more frequently in the granules. GK rats had, in addition to normal-looking islets, some islets with irregular shape and an increased amount of fibrous tissue, so-called "starfish-shaped" islets. In these, beta-granules were usually found, but most of the B-cells were also occupied by large, usually electron-translucent vesicles, some resembling crinophagic bodies, i.e., the sign of intracellular degradation of secretory granules. In starfish-shaped islets, IGF-2 IR was localized to the halo of beta-granules, as in GK islets with normal appearance. Occasionally, IGF-2 IR was also found in the cytoplasm and even in adjacent fibroblasts. Insulin IR was restricted to beta-granules. Because the lysosomes have IGF-2 receptors, the presence of IGF-2 peptide in secretory granules could explain why some granules are guided to lysosomes for degradation.

Accession: 068698339

Download citation: RISBibTeXText

PMID: 9273977

DOI: 10.3109/01913129709021946


Related references

Ultrastructural Localization of Insulin-like Growth Factor-2 (Igf-2) to the Secretory Granules of Insulin Cells: A Study in Normal and Diabetic (Gk) Rats. Ultrastructural Pathology 21(5): 457-466, 1997

Ultrastructural localization of insulin-like growth factor-2 to the secretory granules of insulin cells A study in normal and diabetic rats. Ultrastructural Pathology 21(5): 457-466, 1997

Ultrastructural localization of synaptophysin to the secretory granules of normal glucagon and insulin cells in human islets of Langerhans. Ultrastructural Pathology 15(3): 215-219, 1991

Effect of insulin and insulin like growth factor i on dna synthesis and glucose metabolism in smooth muscle cells from normal and diabetic rats. Acta Endocrinologica Supplementum 89: 4, 1988

Localization of glycoproteins in insulin secretory granules by ultrastructural autoradiography. Journal of Histochemistry and Cytochemistry 35(10): 1059-1062, 1987

Insulin-like growth factor-I, insulin-like growth factor binding protein-3, insulin-like growth factor receptor and urodynamic function after pudendal nerve crush in diabetic female rats. 2008

The relationship between insulin level insulin binding and response to insulin in isolated fat cells from normal ventromedial hypothalamic lesioned and diabetic rats. Acta Physiologica Scandinavica 109(3): 37A, 1980

Effects of recombinant human insulin-like growth factor I and insulin on counterregulation during acute plasma glucose decrements in normal and Type 2 (non-insulin-dependent) diabetic subjects. Diabetologia 36(10): 966-971, 1993

Type IB secretory phospholipase A2 is contained in insulin secretory granules of pancreatic islet beta-cells and is co-secreted with insulin from glucose-stimulated islets. Biochimica et Biophysica Acta 1390(3): 301-312, 1998

Insulin granules. Insulin secretory granules control autophagy in pancreatic β cells. Science 347(6224): 878-882, 2015

Insulin-like growth factor II (IGF-II) inhibits insulin-like growth factor binding protein I (IGFBP-1) production in luteinized human granulosa cells with a potency similar to insulin-like growth factor I (IGF-I) and higher than insulin. Journal of Clinical Endocrinology and Metabolism 81(9): 3412-3414, 1996

Insulin-like growth factor II inhibits insulin-like growth factor binding protein I production in luteinized human granulosa cells with a potency similar to insulin-like growth factor I and higher than insulin. Journal of Clinical Endocrinology and Metabolism 81(9): 3412-3414, 1996

Production of insulin-like growth factor I and its binding protein in rat hepatocytes cultured from diabetic and insulin-treated diabetic rats. Endocrinology 119(5): 2346-2352, 1986

Expression of hepatic insulin-like growth factor-I and insulin-like growth factor-binding protein-1 genes is transcriptionally regulated in streptozotocin-diabetic rats. Molecular Endocrinology 6(6): 969-977, 1992

Expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (Bubalus bubalis) and the effect of insulin-like growth factor I on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro. Theriogenology 83(1): 58-77, 2015