+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein dinB

Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein dinB

Genes to Cells 4(11): 607-618

The Escherichia coli dinB gene is an SOS gene known to be required for lambda phage untargeted mutagenesis. When over-expressed, it exhibits a potent mutagenic activity without any exogenous treatment to damage DNA. Frameshift mutations at a run of identical bases are most enhanced. The product DinB is structurally related to the E. coli UmuC protein and the Saccharomyces cerevisiae Rev1 and Rad30 proteins, all of which are shown to be involved in bypass synthesis at a DNA lesion. We have cloned and sequenced human and mouse cDNAs encoding a DinB homologue. Their products are highly similar to DinB and less similar to UmuC, Rev1 or Rad30, and hence the genes were named DINB1 for human and Dinb1 for mouse. Both genes were expressed most abundantly in testis. Transient expression of the mouse cDNA in cultured mouse cells resulted in a nearly 10-fold increase in the incidence of point mutations, among which about 30% were frameshift mutations. The above results suggest that a mutagenic mechanism, a so-called untargeted type, also operates in mammalian cells. Taken together with recent findings that human cells have multiple DNA polymerases for translesion synthesis which are homologous to the S. cerevisiae Rev3 and Rad30 proteins, our results imply that multiple mutagenic pathways are conserved from bacteria to higher eukaryotes.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 068727026

Download citation: RISBibTeXText

PMID: 10620008

DOI: 10.1046/j.1365-2443.1999.00289.x

Related references

Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein DinB. Genes to Cells 4(11): 607-618, 1999

Mutagenesis by Escherichia coli DinB and its mammalian homolog Pol kappa. Tanpakushitsu Kakusan Koso. Protein Nucleic Acid Enzyme 46(8 Suppl): 1155-1161, 2001

Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA. Proceedings of the National Academy of Sciences of the United States of America 94(25): 13792-7, 1997

Roles of YqjH and YqjW, homologs of the Escherichia coli UmuC/DinB or Y superfamily of DNA polymerases, in stationary-phase mutagenesis and UV-induced mutagenesis of Bacillus subtilis. Journal of Bacteriology 185(7): 2153-2160, 2003

DinB upregulation is the sole role of the SOS response in stress-induced mutagenesis in Escherichia coli. Genetics 182(1): 55-68, 2009

The dinB operon and spontaneous mutation in Escherichia coli. Journal of Bacteriology 185(13): 3972-3977, 2003

Roles of chromosomal and episomal dinB genes encoding DNA pol IV in targeted and untargeted mutagenesis in Escherichia coli. MGG Molecular Genetics & Genomics 266(2): 207-215, 2001

Selection of dinB alleles suppressing survival loss upon dinB overexpression in Escherichia coli. Journal of Bacteriology 196(16): 3023-3035, 2014

Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily. Proceedings of the National Academy of Sciences of the United States of America 96(21): 922-7, 1999

The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism. Genetics 147(4): 1557-1568, 1997

Enhancement of the enzymatic activity of Escherichia coli acetyl esterase by a double mutation obtained by random mutagenesis. Bioscience Biotechnology and Biochemistry 76(11): 2082-2088, 2012

Substitution of fifty four homologue (Ffh) in Escherichia coli with the mammalian 54-kDa protein of signal-recognition particle. European Journal of Biochemistry 238(3): 760-768, 1996

Role of the dinB gene product in spontaneous mutation in Escherichia coli with an impaired replicative polymerase. Journal of Bacteriology 182(23): 6742-6750, 2000

Mutagenesis in Escherichia coli. 3. Requirement for DNA synthesis in mutation by gamma rays of T4-phage complexed with Escherichia coli. Genetical Research 15(2): 147-156, 1970

Influence of experimental conditions and DNA repair ability on EMS-induced mutagenesis and DNA binding in Escherichia coli K12: comparison with mammalian cell mutagenesis. Mutation Research 92(1-2): 15-27, 1982