EurekaMag
+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

A negative regulatory element within the proximal promoter region of the rat ornithine decarboxylase gene


A negative regulatory element within the proximal promoter region of the rat ornithine decarboxylase gene



Molecular Carcinogenesis 29(4): 212-218



ISSN/ISBN: 0899-1987

PMID: 11170259

DOI: 10.1002/1098-2744(200012)29:4<212::aid-mc1003>3.0.co;2-0

A putative Ets site with a core of GGAA located at nt -88 to -85 of the rat ornithine decarboxylase (ODC) gene was characterized by site-directed mutagenesis and transient expression assays. Mutation of this site, when in pODClux2m, which contains a cluster of four Sp1-binding sites, resulted in a 2.6-fold increase in basal promoter activity in untreated cells, whereas the ratio of activity in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated cells relative to the ratio in untreated cells (the induction ratio) remained largely unchanged. However, when the mutation was in pODClux168, which contains only a single Sp1-binding site (GC box V), it caused little alteration to either basal promoter activity or TPA induction ratio. A protein of 55-60 kDa was found specifically bound to this site, as shown by ultraviolet cross-linking assay. In competition assay and methylation interference assay, this protein was shown to occupy the GGAA core, although it showed no antigenic relation to c-Ets-1 in an supershift assay. We suggest that this protein binds specifically to the GGAA core and functions to inhibit activation of the ODC promoter by distal elements, including the upstream Sp1 sites.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 068741774

Download citation: RISBibTeXText

Related references

A novel negative regulatory element in the human collagenase-3 proximal promoter region. Biochemical and Biophysical Research Communications 291(5): 1151-1159, 2002

Identification of a negative regulatory element in the cholesterol 7-alpha-hydroxylase gene proximal promoter. FASEB Journal 9(6): A1334, 1995

Positive and negative regulatory interactions in the rat ornithine decarboxylase promoter. Proceedings of the American Association for Cancer Research Annual Meeting 38: 186, 1997

Identification of a non-canonical E-box motif as a regulatory element in the proximal promoter region of the apolipoprotein E gene. Biochemical Journal 370(Pt 3): 979-986, 2003

A negative regulatory element in the upstream promoter region of the human proopiomelanocortin gene. Van Ree, J M , Et Al (Ed ) International Congress Series, No 914 New Leads in Opioid Research; International Narcotics Research Conference, Noordwijkerhout, Netherlands, July 8-13, Xxvii+391p Elsevier Science Publishers B V (Biomedical Division): Amsterdam, Netherlands; (Dist in The Usa And Canada By Elsevier Science Publishing Co , Inc : New York, New York) Illus 115-117, 1990

Regulation of the expression of human ornithine decarboxylase gene and ornithine decarboxylase promoter-driven reporter gene in transgenic mice. Biochemical Journal 292: 927-932, 1993

Multiple interactions at a regulatory element in the proximal promoter region modulate the AP1 dependent transcription of profilaggrin gene in cultured keratinocytes. Journal of Investigative Dermatology 108(4): 630, 1997

Identification of a bimodal cis-regulatory element encompassing canonical AP-1 binding site in the proximal promoter region of the human decorin gene. Journal of Investigative Dermatology 106(4): 838, 1996

Identification of a bimodal regulatory element encompassing a canonical AP-1 binding site in the proximal promoter region of the human decorin gene. Journal of Biological Chemistry 271(40): 24824-24829, 1996

A negative regulatory element in the promoter region of the rat 2 A-adrenergic receptor gene overlaps an Sp1 consensus binding site. Biochemical Journal 311(2): 541-547, 1995

A negative regulatory element in the promoter region of the rat alpha 2A-adrenergic receptor gene overlaps an SP1 consensus binding site. Biochemical Journal 311: 541-547, 1995

Helicobacter pylori regulates the human histidine decarboxylase promoter through cagA-independent transactivation of a proximal cis-regulatory element. Gastroenterology 118(4 Suppl 2 Pt 1): AGA A741, 2000

Assignment of a gene encoding ornithine decarboxylase to the proximal region of chromosome 12 in the mouse. Biochemical Genetics 27(11-12): 745-753, 1989

HnRNP U interacts with the c-Myc-Max complex on the E-box promoter region inducing the ornithine decarboxylase gene. Oncology Reports 22(2): 249-255, 2009

Protein dna interactions in the cyclic amp responsive promoter region of the murine ornithine decarboxylase gene. Nucleic Acids Research 19(14): 3921-3928, 1991