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Porcine deltacoronavirus induces mitochondrial apoptosis in ST cells



Porcine deltacoronavirus induces mitochondrial apoptosis in ST cells



Sheng Wu Gong Cheng Xue Bao 35(6): 1050-1058



猪丁型冠状病毒 (Porcine deltacoronavirus,PDCoV) 是一种新型的猪肠道致病性冠状病毒,可引起猪群剧烈腹泻及呕吐,但致病机制尚不清楚。本研究检测了PDCoV 感染诱导的细胞凋亡。Caspase 酶活性检测显示,在PDCoV 感染的细胞中,caspase 3、caspase 8 和caspase 9 的活性随病毒感染量的增多而显著提高,类似的现象未能在紫外灭活病毒感染的细胞中观察到,表明PDCoV 感染可同时激活内源性与外源性细胞凋亡通路,并暗示细胞凋亡的诱导依赖于病毒复制。为深入探究PDCoV 诱导的内源性细胞凋亡,分别检测胞浆和线粒体中细胞色素C 与凋亡诱导因子。结果显示,与正常细胞相比,PDCoV 感染细胞从线粒体释放到胞浆的细胞色素C 显著增多,且其释放量随着感染时间的延长而增多,而凋亡诱导因子始终定位于线粒体,提示PDCoV 感染通过促使线粒体膜间隙的细胞色素C 进入胞浆而启动caspase 依赖的线粒体凋亡通路。本研究初步揭示了PDCoV 诱导细胞凋亡的机制。.

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Accession: 069084467

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PMID: 31232001


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