+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells



Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells



Virus Genes 55(6): 795-801



Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus that causes intestinal diseases in neonatal piglets with diarrhea, vomiting, dehydration, and post-infection mortality of 50-100%. Currently, there are no effective treatments or vaccines available to control PDCoV. To study the potential of RNA interference (RNAi) as a strategy against PDCoV infection, two short hairpin RNA (shRNA)-expressing plasmids (pGenesil-M and pGenesil-N) that targeted the M and N genes of PDCoV were constructed and transfected separately into swine testicular (ST) cells, which were then infected with PDCoV strain HB-BD. The potential of the plasmids to inhibit PDCoV replication was evaluated by cytopathic effect, virus titers, and real-time quantitative RT-PCR assay. The cytopathogenicity assays demonstrated that pGenesil-M and pGenesil-N protected ST cells against pathological changes with high specificity and efficacy. The 50% tissue culture infective dose showed that the PDCoV titers in ST cells treated with pGenesil-M and pGenesil-N were reduced 13.2- and 32.4-fold, respectively. Real-time quantitative RT-PCR also confirmed that the amount of viral RNA in cell cultures pre-transfected with pGenesil-M and pGenesil-N was reduced by 45.8 and 56.1%, respectively. This is believed to be the first report to show that shRNAs targeting the M and N genes of PDCoV exert antiviral effects in vitro, which suggests that RNAi is a promising new strategy against PDCoV infection.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069287977

Download citation: RISBibTeXText

PMID: 31463771


Related references

Inhibition of porcine hemagglutinating encephalomyelitis virus replication by short hairpin RNAs targeting of the nucleocapsid gene in a porcine kidney cell line. Journal of Virological Methods 179(2): 414-418, 2012

Inhibition of porcine transmissible gastroenteritis virus infection in porcine kidney cells using short hairpin RNAs targeting the membrane gene. Virus Genes 53(2): 226-232, 2017

In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene. Virology Journal 9: 176, 2012

Specific inhibition of HIV-1 replication by short hairpin RNAs targeting human cyclin T1 without inducing apoptosis. Febs Letters 579(14): 3100-3106, 2005

Argonaute-2 enhances suppression of human cytomegalovirus replication by polycistronic short hairpin RNAs targeting UL46, UL70 and UL122. Antiviral Therapy 16(5): 741-749, 2011

Down-regulation of viral replication by lentiviral-mediated expression of short-hairpin RNAs against vesicular stomatitis virus ribonuclear complex genes. Antiviral Research 95(2): 150-158, 2012

Short hairpin RNAs with a 2- or 3-base mismatch inhibit HBV expression and replication in HepG2 cells. Hepatology International 7(1): 127-133, 2013

Comparison of inhibitory efficacy of short interfering RNAs targeting different genes on Measles virus replication. Journal of Basic Microbiology 52(3): 332-339, 2012

C gene-targeting short hairpin RNA suppresses the replication and expression of hepatitis B virus in BHK-21 cells. Zhonghua Yi Xue Za Zhi 90(39): 2776-2781, 2010

Inhibition of porcine reproductive and respiratory syndrome virus replication by short hairpin RNA in MARC-145 cells. Veterinary Microbiology 115(4): 302-310, 2006

Effective suppression of replication of porcine reproductive and respiratory syndrome virus by adenovirus-mediated small interfering RNAs targeting ORF1b, 5 and 7 genes. Journal of Virological Methods 157(1): 40-46, 2009

Effective inhibition of HIV-1 production by short hairpin RNAs and small interfering RNAs targeting a highly conserved site in HIV-1 Gag RNA is optimized by evaluating alternative length formats. Antimicrobial Agents and ChemoTherapy 59(9): 5297-5305, 2015

Human H1 promoter expressed short hairpin RNAs (shRNAs) suppress avian influenza virus replication in chicken CH-SAH and canine MDCK cells. Antiviral Research 84(2): 159-167, 2009

Short communication: Efficiently inhibiting HIV-1 replication by a prototype foamy virus vector expressing novel H1 promoter-driven short hairpin RNAs. Aids Research and Human Retroviruses 31(2): 183-188, 2015

Selective inhibition of enterovirus 71 replication by short hairpin RNAs. Biochemical and Biophysical Research Communications 325(2): 494-499, 2004