+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of peptide domains involved in the subcellular localization of the feline coronavirus 3b protein



Identification of peptide domains involved in the subcellular localization of the feline coronavirus 3b protein



Journal of General Virology 100(10): 1417-1430



Feline coronavirus (FCoV) has been identified as the aetiological agent of feline infectious peritonitis (FIP), a highly fatal systemic disease in cats. FCoV open reading frame 3 (ORF3) encodes accessory proteins 3a, 3b and 3 c. The FCoV 3b accessory protein consists of 72 amino acid residues and localizes to nucleoli and mitochondria. The present work focused on peptide domains within FCoV 3b that drive its intracellular trafficking. Transfection of different cell types with FCoV 3b fused to enhanced green fluorescent protein (EGFP) or 3×FLAG confirmed localization of FCoV 3b in the mitochondria and nucleoli. Using serial truncated mutants, we showed that nucleolar accumulation is controlled by a joint nucleolar and nuclear localization signal (NoLS/NLS) in which the identified overlapping pat4 motifs (residues 53-57) play a critical role. Mutational analysis also revealed that mitochondrial translocation is mediated by N-terminal residues 10-35, in which a Tom20 recognition motif (residues 13-17) and two other overlapping hexamers (residues 24-30) associated with mitochondrial targeting were identified. In addition, a second Tom20 recognition motif was identified further downstream (residues 61-65), although the mitochondrial translocation evoked by these residues seemed less efficient as a diffuse cytoplasmic distribution was also observed. Assessing the spatiotemporal distribution of FCoV 3b did not provide convincing evidence of dynamic shuttling behaviour between the nucleoli and the mitochondria.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069305168

Download citation: RISBibTeXText

PMID: 31483243


Related references

Domains of the TMV movement protein involved in subcellular localization. Plant Journal 15(1): 15-25, 1998

Identification of RCNMV movement protein domains involved in virus cell-to-cell movement, subcellular localization, and intermolecular complementation. Phytopathology 91(6 Suppl.): S89, 2001

First identification of a single amino acid change in the spike protein region of feline coronavirus detected from a coronavirus-associated cutaneous nodule in a cat. Jfms Open Reports 4(2): 2055116918801385, 2018

Identification of amino acid residues and protein kinases involved in the regulation of NFATc subcellular localization. Journal of Biological Chemistry 275(5): 3543-3551, 2000

Peptide domains involved in the localization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus. Virology 316(1): 135-145, 2003

Identification of the amino acid residues and domains in the cysteine-rich protein of Chinese wheat mosaic virus that are important for RNA silencing suppression and subcellular localization. Molecular Plant Pathology 14(3): 265-278, 2013

Protein kinase C epsilon is localized to the Golgi Identification of PKC-epsilon subcellular localization domains and proteolytic degradation sites. FASEB Journal 9(6): A1408, 1995

Spike protein fusion peptide and feline coronavirus virulence. Emerging Infectious Diseases 18(7): 1089-1095, 2012

Synthesis and subcellular localization of the murine coronavirus nucleocapsid protein. Virology 130(2): 527-532, 1983

Mapping of the coronavirus membrane protein domains involved in interaction with the spike protein. Journal of Virology 73(9): 7441-7452, 1999

Identification and subcellular localization of a 41 kDa, polyprotein 1ab processing product in human coronavirus 229E-infected cells. Journal of General Virology 78: 2789-2794, 1997

Domains of the human androgen receptor involved in steroid binding, transcriptional activation, and subcellular localization. Molecular Endocrinology 5(10): 1396-1404, 1991

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein. Journal of General Virology 86(Pt 12): 3303-3310, 2005

Identification of Ourmiavirus 30K movement protein amino acid residues involved in symptomatology, viral movement, subcellular localization and tubule formation. Molecular Plant Pathology 17(7): 1063-1079, 2016

Identification and characterization of a Golgi retention signal in feline coronavirus accessory protein 7b. Journal of General Virology 98(8): 2017-2029, 2017