EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Excystation of Eimeria acervulina, E. maxima, and E. tenella differs in response to trypsin and chymotrypsin and the presence of reducing agents DTT and TCEP


Excystation of Eimeria acervulina, E. maxima, and E. tenella differs in response to trypsin and chymotrypsin and the presence of reducing agents DTT and TCEP



Molecular and Biochemical Parasitology 233: 111219



ISSN/ISBN: 0166-6851

PMID: 31518610

DOI: 10.1016/j.molbiopara.2019.111219

Release of sporozoites from Eimeria oocysts/sporocysts is an essential step in the intracellular development of the parasite in its host. Little is known about this process except that elevated temperature (∼ 40 °C) plus trypsin and bile salts are required for sporozoite to escape from sporocysts. In this study, it was found that adding a reducing agent, either dithiothreitol (DTT) or Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), increased the lifespan of sporozoites released from Eimeria maxima. While the addition of DTT or TCEP affected the apparent molecular weight of trypsin, it did not interfere with excystation of E. maxima, but rather had a positive effect on the number of viable sporozoites present after release. This effect was time-dependent in that the number of intact sporozoites at 15 and 30 min after excystation was similar between untreated and DTT- or TCEP-treated sporocysts. However, by 45-60 min, virtually no sporozoites were observed in excystation fluid not containing DTT or TCEP. Of interest is that this effect appeared to be Eimeria species-dependent. Eimeria acervulina and E. tenella sporozoites remained viable for at least 60 min after excystation in the absence of DTT or TCEP. The effect of DTT and TCEP on chymotrypsin was also studied with all 3 Eimeria species because there is some evidence that chymotrypsin is an effective excystation enzyme. Indeed, E. maxima sporozoites excysting from sporocysts with chymotrypsin in the presence of DTT or TCEP remained viable for at least 60 min after release, unlike excystation done in the absence of these reducing agents. Chymotrypsin was capable of excysting E. acervulina in the presence or absence of DTT or TCEP. Of interest, is that chymotrypsin was ineffective in the excystation of E. tenella. These findings suggest that trypsin and chymotrypsin have differential effects on sporozoite excystation and that reducing agents may alter sites on the enzyme that affect sporozoite viability, but not release from sporocysts.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 069336608

Download citation: RISBibTeXText

Related references

Role of chicken pancreatic trypsin, chymotrypsin and elastase in the excystation process of Eimeria tenella oocysts and sporocysts. Journal of Protozoology Research 1(15): 22-26, 1991

Titration of anticoccidial activity of salinomycin in six battery experiments with replacement pullets infected with single and mixed field strain isolates of eimeria necatrix eimeria acervulina eimeria mivati eimeria tenella eimeria maxima and eimeria brunetti. Poultry Science 65(Suppl. 1): 194, 1986

Induction of protective immunity against Eimeria tenella, Eimeria necatrix, Eimeria maxima and Eimeria acervulina infections using multivalent epitope DNA vaccines. Vaccine 33(24): 2764-2770, 2015

Cytochemical observations on sporozoites of chicken coccidia, Eimeria acervulina, Eimeria hagani, Eimeria maxima, and Eimeria tenella. Japanese journal of veterinary science = Nihon juigaku zasshi 43(5): 767-769, 1981

Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella, Eimeria acervulina and Eimeria maxima. Bmc Veterinary Research 14(1): 337, 2018

Anti coccidial activity of sulfadimethoxine anti parasit potentiated mixture in chickens eimeria tenella eimeria necatrix eimeria acervulina eimeria maxima eimeria branetti eimeria mivati. Poultry Science: 210-216, 1969

Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis. Oncotarget 8(21): 34935-34945, 2017

Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina. International Journal for Parasitology 39(10): 1063-1070, 2009

Coccidian Merozoite Transcriptome Analysis From Eimeria Maxima In Comparison To Eimeria Tenella And Eimeria Acervulina. The Journal of Parasitology 96(1): 49-57, 2010

Coccidian merozoite transcriptome analysis from Eimeria maxima in comparison to Eimeria tenella and Eimeria acervulina. Journal of Parasitology 96(1): 49-57, 2010

Effects of different levels of oocyst inocula eimeria acervulina eimeria tenella and eimeria maxima on plasma constituents in chickens. Poultry Science 70(Suppl. 1): 29, 1991

Anticoccidial activity of 8 aminoquinolines pamaquine primaquine and several molecular complexes and salts of pamaquine against eimeria tenella eimeria necatrix eimeria acervulina eimeria maxima and eimeria brunetti in battery experiments. Journal of Veterinary Medical Science 53(1): 13-18, 1991

Differing susceptibilities of Eimeria acervulina, Eimeria maxima, and Eimeria tenella oocysts to desiccation. Journal of Parasitology 99(5): 899-902, 2013

Some observations on the development of Eimeria tenella, Eimeria acervulina and Eimeria maxima in a non-specific host. Research in avian coccidiosis: proceedings of the Georgia Coccidiosis Conference November 19-21-1985 edited by Larry R McDougald Leonard P Joyner Peter L Long: 4, 1986

Differing Susceptibilities of Eimeria acervulina, Eimeria maxima, and Eimeria tenella Oocysts to Desiccation. The Journal of Parasitology 99(5): 899-902, 2013