EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress


Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress



Genes and Genomics 41(12): 1445-1455



ISSN/ISBN: 1976-9571

PMID: 31535316

DOI: 10.1007/s13258-019-00870-2

Snap bean, Phaseolus vulgaris L., as a warm-season vegetable, low temperature stress seriously affect the yield and quality. At present, little is known about the genes and molecular regulation mechanism in cold response in snap bean exposed to low temperature. Our objectives were to identify the low temperature response genes in snap bean and to examine differences in the gene response between cold-tolerant and cold-sensitive genotypes. We used two highly inbred snap bean lines in this study, the cold-tolerant line '120', and the cold-sensitive line '093'. The plants were grown to the three leaf and one heart stage and exposed to 4 °C low temperature. We used RNA sequencing (RNA-seq) to analyze the differences of gene expression. 988 and 874 cold-responsive genes were identified in 'T120 vs CK120' and 'T093 vs CK093' ('T' stands for low temperature treatment, and 'CK' stands for control at room temperature), respectively. Of these, 555 and 442 genes were unique to cold-stressed lines '120' and '093', respectively compared to the control. Our analysis of these differentially expressed genes indicates that Ca2+, ROS, and hormones act as signaling molecules that play important roles in low temperature response in P. vulgaris. Altering the expression of genes in these signaling pathways activates expression of downstream response genes which can interact with other signaling regulatory networks. This may maintained the balance of ROS and hormones, making line '120' more cold-tolerant than line '093'. Our results provide a preliminarily understanding of the molecular basis of low temperature response in snap bean, and also establish a foundation for the future genetic improvement of cold sensitivity in snap bean by incorporating genes for cold tolerance.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 069351166

Download citation: RISBibTeXText

Related references

Comparative Transcriptome Analysis Reveals Differential Response of Phytohormone Biosynthesis Genes in Glumous Flowers of Cold-Tolerant and Cold-Sensitive Rice Varieties Upon Cold Stress at Booting Stage. Korean Journal of Breeding Science 43(1): 1-13, 2011

Effects of temperature on the vegetative and early reproductive growth of a cold-tolerant and a cold-sensitive line of nitrate-fed Phaseolus vulgaris L. Crop Research 27(1): 49-60, 1987

Effect of temperature on growth nodulation xylem sap composition nodule uricase and allantoinase activities in a cold tolerant and a cold sensitive line of phaseolus vulgaris. Plant Physiology 69(4 Suppl): 122, 1982

Rhizoplane iron stress response physiology of normal snap bean phaseolus vulgaris seedlings vs. temperature sensitive chlorotic hybrids. Plant Physiology 75(Suppl 1): 95, 1984

The Effects of Temperature on Vegetative and Early Reproductive Growth of a Cold-Tolerant and a Cold-Sensitive Line of Phaseolus vulgaris L. 2. Nodular Uricase, Allantoinase, Xylem transport of N and Assimilation in Shoot Tissues. Annals of Botany 53(4): 589-597, 1984

The effects of temperature on vegetative and early reproductive growth of a cold-tolerant and a cold-sensitive line of Phaseolus vulgaris L. 2. Nodular uricase, allantoinase, xylem transport of N and assimilation in shoot tissues. Annals of Botany 53(4): 589-597, 1984

The effects of temperature on vegetative and early reproductive growth of a cold tolerant and a cold sensitive line of phaseolus vulgaris 2. nodular uricase allantoinase xylem transport of nitrogen and assimilation in shoot tissues. Annals of Botany 53(4): 589-598, 1984

The Effects of Temperature on Vegetative and Early Reproductive Growth of a Cold-Tolerant and, a Cold-Sensitive Line of Phaseolus vulgaris L. 1. Nodulation, Growth and Partitioning of Dry Matter, Carbon and Nitrogen. Annals of Botany 53(4): 579-588, 1984

The effects of temperature on vegetative and early reproductive growth of a cold tolerant and a cold sensitive line of phaseolus vulgaris 1. nodulation growth and partitioning of dry matter carbon and nitrogen. Annals of Botany 53(4): 579-588, 1984

Differential Effects of Cold Stress on Chloroplasts Structures and Photosynthetic Characteristics in Cold-Sensitive and Cold-Tolerant Cultivars of Sugarcane. Sugar Tech 20(1): 11-20, 2018

Comparison of the gene expression related to cold acclimation between the freezing-tolerant and sensitive barley varieties by differential display. Korean Journal of Genetics 26(4): 351-358, 2004

Water status of flower buds and leaves as affected by high temperature in heat-tolerant and heat-sensitive cultivars of snap bean (Phaseolus vulgaris L.). Plant Production Science 6(1): 24-27, 2003

Influence of water and high temperature stresses on leaf water status of high temperature tolerant and sensitive cultivars of snap bean (Phaseolus vulgaris L.). Japanese Journal of Tropical Agriculture 49(2): 109-118, 2005

Transcriptomic analysis of Litopenaeus vannamei hepatopancreas under cold stress in cold-tolerant and cold-sensitive cultivars. Gene 764: 145090, 2021

Comparative transcriptome analysis of RNA-seq data for cold-tolerant and cold-sensitive rice genotypes under cold stress. Journal of Plant Biology 57(6): 337-348, 2014