+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genetic Analysis of Avian Coronavirus Infectious Bronchitis Virus in Yellow Chickens in Southern China over the Past Decade: Revealing the Changes of Genetic Diversity, Dominant Genotypes, and Selection Pressure



Genetic Analysis of Avian Coronavirus Infectious Bronchitis Virus in Yellow Chickens in Southern China over the Past Decade: Revealing the Changes of Genetic Diversity, Dominant Genotypes, and Selection Pressure



Viruses 11(10)



The high mutation rates of infectious bronchitis virus (IBV) pose economic threats to the poultry industry. In order to track the genetic evolutionary of IBV isolates circulating in yellow chickens, we continued to conduct the genetic analyses of the structural genes S1, E, M, and N from 64 IBV isolates in southern China during 2009-2017. The results showed that the dominant genotypes based on the four genes had changed when compared with those during 1985-2008. Based on the S1 gene phylogenetic tree, LX4-type (GI-19) was the most dominant genotype, which was different from that during 1985-2008. The second most dominant genotype was LDT3-A-type, but this genotype disappeared after 2012. New-type 1 (GVI-1) isolates showed increasing tendency and there were four aa (QKEP) located in the hypervariable region (HVR) III and one aa (S) insertion in all the New-type 1 isolates. Both the analyses of amino acid entropy and molecular evolutionary rate revealed that the variations from large to small were S1, E, M, and N. Purifying selection was detected in the S1, E, M, and N gene proteins, which was different from the positive selection during 1985-2008. Six isolates were confirmed to be recombinants, possibly generated from a vaccine virus of the 4/91-type or LDT3-A-type and a circulating virus. The estimated times for the most recent common ancestors based on the S1, E, M, and N genes were the years of 1744, 1893, 1940, and 1945, respectively. Bayesian skyline analysis revealed a sharp decrease in genetic diversity of all the four structural genes after 2010 and since late 2015, the viral population rapidly rose. In conclusion, the IBVs circulating in southern China over the past decade have experienced a remarkable change in genetic diversity, dominant genotypes, and selection pressure, indicating the importance of permanent monitoring of circulating strains and the urgency for developing new vaccines to counteract the emerging LX4-type and New-type IBVs.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069374059

Download citation: RISBibTeXText

PMID: 31561498


Related references

Genetic diversity of avian infectious bronchitis coronavirus in recent years in China. Avian Diseases 56(1): 15-28, 2012

Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between 1995 and 2004. Archives of Virology 151(6): 1133-1148, 2006

Genetic diversity of avian infectious bronchitis virus in China in recent years. Infection Genetics and Evolution 66: 82-94, 2018

Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection. Journal of Virological Methods 123(2): 203-211, 2005

Molecular characterization of major structural protein genes of avian coronavirus infectious bronchitis virus isolates in southern china. Viruses 5(12): 3007-3020, 2013

Analysis of the S1 gene of the avian infectious bronchitis virus (IBV) reveals changes in the IBV genetic groups circulating in southern Thailand. Research in Veterinary Science 100: 299-302, 2015

Phylodynamic analysis and molecular diversity of the avian infectious bronchitis virus of chickens in Brazil. Infection, Genetics and Evolution 61: 77-83, 2018

Phylodynamic analysis and molecular diversity of the avian infectious bronchitis virus of chickens in Brazil. Infection Genetics and Evolution 61: 77-83, 2018

Isolation and genetic analysis revealed no predominant new strains of avian infectious bronchitis virus circulating in South China during 2004-2008. Veterinary Microbiology 143(2-4): 145-154, 2010

Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area, China. Virus Genes 41(2): 202-209, 2010

Genetic and Antigenic Diversity in Avian Infectious Bronchitis Virus Isolates of the 1940s. Avian Diseases 46(2): 437-441, 2002

Genetic and antigenic diversity in avian infectious bronchitis virus isolates of the 1940s. Avian Diseases 46(2): 437-441, 2002

Interferon induction by several strains of avian infectious bronchitis virus, a coronavirus, in chickens. Acta Virologica 32(1): 55-59, 1988

Genetic diversity of avian infectious bronchitis virus isolates in Korea between 2003 and 2006. Avian Diseases 52(2): 332-337, 2008

Genetic diversity and selection regulates evolution of infectious bronchitis virus. Avian Diseases 56(3): 449-455, 2012