+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcriptome analysis of PK-15 cells in innate immune response to porcine deltacoronavirus infection

Transcriptome analysis of PK-15 cells in innate immune response to porcine deltacoronavirus infection

Plos one 14(10): E0223177

Porcine deltacoronavirus (PDCoV) is a newly emerged swine enteropathogenic coronavirus affecting pigs of all ages and causing diarrhea problems. Research findings indicate that PDCoV has evolved strategies to escape innate immune response in host cells, but mechanism of PDCoV in innate immune modulation is not well understood. In this study, we report our findings on identifying the alterations of host cell innate immune response affected by PDCoV infection and exploring the gene expression profiles of PK-15 cells at 0, 24, and 36 h PDCoV post infection by RNA sequencing. A total of 3,762 and 560 differentially expressed genes (DEGs) were screened by comparison of uninfected PK-15 cells and infected PK-15 cells at 24 h post infection (hpi) (INF_24h versus NC), and also comparison of infected PK-15 cells between 24 and 36 hpi (INF_36h versus INF_24h), which included 156 and 23 porcine innate immune-related genes in the DEGs of INF_24h versus NC and INF_36h versus INF_24h, respectively. Gene Ontology function classification and Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment analysis were performed based on the DEGs that exhibited the same expression tendencies with most of the innate immune-associated genes among these PK-15 cell samples described above. The enrichment results indicated that extensive gene functions and signaling pathways including innate immune-associated functions and pathways were affected by PDCoV infection. Particularly, 4 of 5 innate immune signaling pathways, which were primarily affected by PDCoV, played important roles in I-IFN's antiviral function in innate immune response. Additionally, 16 of the host cell endogenous miRNAs were predicted as potential contributors to the modulation of innate immune response affected by PDCoV. Our research findings indicated that the innate immune-associated genes and signaling pathways in PK-15 cells could be modified by the infection of PDCoV, which provides a fundamental foundation for further studies to better understand the mechanism of PDCoV infections, so as to effectively control and prevent PDCoV-induced swine diarrheal disease outbreaks.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 069385266

Download citation: RISBibTeXText

PMID: 31574122

Related references

Global transcriptome analysis of Staphylococcus aureus biofilms in response to innate immune cells. Infection and Immunity 81(12): 4363-4376, 2013

MicroRNA-146 function in the innate immune transcriptome response of zebrafish embryos to Salmonella typhimurium infection. Bmc Genomics 14: 696, 2013

Transcriptome profiling and functional analyses of the zebrafish embryonic innate immune response to Salmonella infection. Journal of Immunology 182(9): 5641-5653, 2009

Transcriptome analysis of Traf6 function in the innate immune response of zebrafish embryos. Molecular Immunology 48(1-3): 179-190, 2010

Specificity of the zebrafish host transcriptome response to acute and chronic mycobacterial infection and the role of innate and adaptive immune components. Molecular Immunology 46(11-12): 2317-2332, 2009

Porcine (Sde(B T cells: Possible roles on the innate and adaptive immune responses following virus infection. Veterinary immunology and immunopathology 15 112(1-2): 49-61, 2006

Susceptibility of porcine Ipec-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PdcoV) and serum cytokine responses of gnotobiotic pigs to acute infection with Ipec-J2 cell culture-passaged PdcoV. Veterinary Microbiology 221: 49-58, 2018

Porcine gammadelta T cells: possible roles on the innate and adaptive immune responses following virus infection. Veterinary Immunology and Immunopathology 112(1-2): 49-61, 2006

Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus as a model to study innate immune responses to enteric viruses. Archives of Virology 158(7): 1467-1475, 2013

Analysis of long non-coding RNAs in neonatal piglets at different stages of porcine deltacoronavirus infection. Bmc Veterinary Research 15(1): 111, 2019

Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response. Bmc Genomics 11: 292, 2010

Gene expression analysis during dengue virus infection in HepG2 cells reveals virus control of innate immune response. Journal of Infection 60(1): 65-75, 2010

The immune response in rohu, Labeo rohita (Actinopterygii: Cyprinidae) to Argulus siamensis (Branchiura: Argulidae) infection: kinetics of immune gene expression and innate immune response. Aquaculture Research 46(6): 1292-1308, 2015

Candida albicans airway exposure primes the lung innate immune response against Pseudomonas aeruginosa infection through innate lymphoid cell recruitment and interleukin-22-associated mucosal response. Infection and Immunity 82(1): 306-315, 2014

Calves are susceptible to infection with the newly emerged porcine deltacoronavirus, but not with the swine enteric alphacoronavirus, porcine epidemic diarrhea virus. Archives of Virology 162(8): 2357-2362, 2017