+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Porcine deltacoronavirus (PDCoV) modulates calcium influx to favor viral replication



Porcine deltacoronavirus (PDCoV) modulates calcium influx to favor viral replication



Virology 539: 38-48



Ionic calcium (Ca2+) is a versatile intracellular second messenger that plays important roles in cellular physiological and pathological processes. Porcine deltacoronavirus (PDCoV) is an emerging enteropathogenic coronavirus that causes serious vomiting and diarrhea in suckling piglets. In this study, the role of Ca2+ to PDCoV infection was investigated. PDCoV infection was found to upregulate intracellular Ca2+ concentrations of IPI-2I cells. Chelating extracellular Ca2+ by EGTA inhibited PDCoV replication, and this inhibitory effect was overcome by replenishment with CaCl2. Treatment with Ca2+ channel blockers, particularly the L-type Ca2+ channel blocker diltiazem hydrochloride, inhibited PDCoV infection significantly. Mechanistically, diltiazem hydrochloride reduces PDCoV infection by inhibiting the replication step of the viral replication cycle. Additionally, knockdown of CACNA1S, the L-type Ca2+ voltage-gated channel subunit, inhibited PDCoV replication. The combined results demonstrate that PDCoV modulates calcium influx to favor its replication.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069468015

Download citation: RISBibTeXText

PMID: 31670218


Related references

Susceptibility of porcine Ipec-J2 intestinal epithelial cells to infection with porcine deltacoronavirus (PdcoV) and serum cytokine responses of gnotobiotic pigs to acute infection with Ipec-J2 cell culture-passaged PdcoV. Veterinary Microbiology 221: 49-58, 2018

Characteristics of the Life Cycle of Porcine Deltacoronavirus (PDCoV) In Vitro: Replication Kinetics, Cellular Ultrastructure and Virion Morphology, and Evidence of Inducing Autophagy. Viruses 11(5):, 2019

Herd-level prevalence and incidence of porcine epidemic diarrhoea virus (PEDV) and porcine deltacoronavirus (PDCoV) in swine herds in Ontario, Canada. Transboundary and Emerging Diseases 65(5): 1197-1207, 2018

Porcine deltacoronavirus (PDCoV) infection suppresses RIG-I-mediated interferon-β production. Virology 495: 10-17, 2016

Identification of an amino acid domain encoded by the capsid gene of porcine circovirus type 2 that modulates intracellular viral protein distribution during replication. Virus Research 155(1): 358-362, 2011

Hepatitis B virus modulates store-operated calcium entry to enhance viral replication in primary hepatocytes. Plos one 12(2): E0168328, 2017

A calcium-induced calcium influx factor, nitric oxide, modulates the refilling of calcium stores in astrocytes. Journal of Neuroscience 23(32): 10302-10310, 2003

Morphological studies of viral infections of the intestinal tract-viral replication and cytopathology in cell cultures and in piglet enterocytes. 3. Replication of porcine adenoviruses in cell culture in comparison with rotaviruses and coronaviruses. Archiv fur Experimentelle Veterinarmedizin 42(4): 584-594, 1988

Sarcoplasmic reticulum directly buffers calcium influx via the sarcolemmal sodium-calcium exchanger and rapidly modulates its impact on calcium release in cardiac myocytes. Biophysical Journal 72(2 Part 2): A163, 1997

MicroRNA-like viral small RNA from porcine reproductive and respiratory syndrome virus negatively regulates viral replication by targeting the viral nonstructural protein 2. Oncotarget 7(50): 82902-82920, 2016

Porcine MKRN1 Modulates the Replication and Pathogenesis of Porcine Circovirus Type 2 by Inducing Capsid Protein Ubiquitination and Degradation. Journal of Virology 92(11):, 2018

Correction for Wang et al., "Porcine MKRN1 Modulates the Replication and Pathogenesis of Porcine Circovirus Type 2 by Inducing Capsid Protein Ubiquitination and Degradation". Journal of Virology 92(21):, 2018

Calcium sequestering ability of mitochondria modulates influx of calcium through glutamate receptor channel. Neurochemical Research 25(12): 1527-1536, 2000

Two-way antigenic cross-reactivity between porcine epidemic diarrhea virus and porcine deltacoronavirus. Veterinary Microbiology 186: 90-96, 2016

Action potential duration modulates calcium influx, Na(+)-Ca2+ exchange, and intracellular calcium release in rat ventricular myocytes. Annals of the new York Academy of Sciences 779: 417-429, 1996