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Off-line comprehensive two-dimensional reversed-phase countercurrent chromatography with high-performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc


Off-line comprehensive two-dimensional reversed-phase countercurrent chromatography with high-performance liquid chromatography: Orthogonality in separation of Polygonum cuspidatum Sieb. et Zucc



References 43(3): 561-568



ISSN/ISBN: 1615-9306

PMID: 31675760

DOI: 10.1002/jssc.201900877

Off-line comprehensive two-dimensional reversed-phase countercurrent chromatography with high-performance liquid chromatography was investigated in separation of crude ethanol extract from traditional Chinese medicinal herb Polygonum cuspidatum Sieb. et Zucc. Two-dimensional contour plots for countercurrent chromatography with high-performance liquid chromatography was obtained after comprehensive separation was completed. Total peak capacity was evaluated and approximately 810 peaks were obtained through a comprehensive two-dimensional separation. A highly orthogonality of 52.23% and a large separation space occupancy of 88.86% were achieved. Meanwhile, it was found that several components could be well separated by countercurrent chromatography while they could not be separated by high-performance liquid chromatography, and vice versa, which further indicated the orthogonality of the two separation methods. The off-line comprehensive two-dimensional countercurrent chromatography with high-performance liquid chromatography provided a promising and powerful method for separation of complex natural products.

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Accession: 069472912

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