+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

GABA synthesis mediated by γ-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and α-ketoglutarate decarboxylase genes



GABA synthesis mediated by γ-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and α-ketoglutarate decarboxylase genes



Plant Science 290: 110287



A pathway for polyamine-derived GABA synthesis in Synechocystis sp. PCC 6803 was explored by disrupting both the glutamate decarboxylase and α-ketoglutarate decarboxylase genes. The generated Δgad:Δkgd strain had increased intracellular α-ketoglutarate and polyamine levels compared to the wild type. Gene transcript analysis using RT-PCR indicated that the Δgad:Δkgd strain had up-regulated expression of a putative gadbh whose gene product, γ-aminobutanal dehydrogenase, would catalyze the conversion of γ-aminobutanal to GABA. A strain with disrupted gabdh showed an increase in GABA, glutamate, succinate and spermidine levels. These findings provide evidence for a link between spermidine degradation and GABA synthesis in cyanobacteria. This study highlights the role of γ-aminobutanal dehydrogenase in maintaining an intact tricarboxylic acid cycle in Synechocystis.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069564283

Download citation: RISBibTeXText

PMID: 31779897


Related references

Characterization of glutamate decarboxylase from Synechocystis sp. PCC6803 and its role in nitrogen metabolism. Plant Physiology and Biochemistry 99: 59-65, 2016

Construction of sll0862 or slr0643 disrupted mutants and their phenotype analysis in Synechocystis sp. PCC6803. Wei Sheng Wu Xue Bao 49(11): 1470-1476, 2009

Modulation of cellular glutamate levels by adenovirus mediated over-expression of glutamate dehydrogenase or glutamate decarboxylase. Diabetes 50(Suppl. 2): A357, 2001

Alpha-ketoglutarate dehydrogenase and glutamate dehydrogenase work in tandem to modulate the antioxidant alpha-ketoglutarate during oxidative stress in Pseudomonas fluorescens. Journal of Bacteriology 191(12): 3804-3810, 2009

GAD65 and GABA-transaminase genes are more highly expressed in pancreatic islets than the alpha-ketoglutarate dehydrogenase gene. 2007

Glutamate dehydrogenase, glutamic acid decarboxylase, and GABA amino transferase in epileptic mouse cortex. Canadian Journal of Physiology and Pharmacology 52(5): 952-959, 1974

Chorismate pyruvate-lyase and 4-hydroxy-3-solanesylbenzoate decarboxylase are required for plastoquinone biosynthesis in the cyanobacterium Synechocystis sp. PCC6803. Journal of Biological Chemistry 289(5): 2675-2686, 2014

Properties of the respiratory NAD(P)H dehydrogenase isolated from the cyanobacterium Synechocystis PCC6803. Plant & Cell Physiology 39(3): 263-267, 1998

Separation of hydrophobic NAD(P)H dehydrogenase subcomplexes from cyanobacterium Synechocystis PCC6803. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica 35(8): 723-727, 2003

Experimental evidence that maleic acid markedly compromises glutamate oxidation through inhibition of glutamate dehydrogenase and α-ketoglutarate dehydrogenase activities in kidney of developing rats. MolecularandCellularBiochemistry458(1-2):99, 2019

Using codons in Synechocystis PCC6803 genes. Fiziologiya Rastenii 41(2): 163-169, 1994

Response of NAD(P)H dehydrogenase complex to the alteration of CO2 concentration in the cyanobacterium Synechocystis PCC6803. Journal of Plant Physiology 160(8): 967-970, 2003

Menaquinone (vitamin K2) biosynthesis in Escherichia coli: synthesis of o-succinylbenzoate does not require the decarboxylase activity of the ketoglutarate dehydrogenase complex. Biochemistry 25(6): 1304-1307, 1986

The gene encoding the NdhH subunit of type 1 NAD(P)H dehydrogenase is essential to survival of synechocystis PCC6803. Febs Letters 487(2): 272-276, 2000

Effects of low CO2 on NAD(P)H dehydrogenase, a mediator of cyclic electron transport around photosystem I in the cyanobacterium synechocystis PCC6803. Plant & Cell Physiology 44(5): 534-540, 2003