+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Proteome-Wide Identification of Lysine Propionylation in the Conidial and Mycelial Stages of Trichophyton rubrum



Proteome-Wide Identification of Lysine Propionylation in the Conidial and Mycelial Stages of Trichophyton rubrum



Frontiers in Microbiology 10: 2613



Posttranslational modifications (PTMs) exist in a wide variety of organisms and play key roles in regulating various essential biological processes. Lysine propionylation is a newly discovered PTM that has rarely been identified in fungi. Trichophyton rubrum (T. rubrum) is one of the most common fungal pathogens in the world and has been studied as an important model organism of anthropic pathogenic filamentous fungi. In this study, we performed a proteome-wide propionylation analysis in the conidial and mycelial stages of T. rubrum. A total of 157 propionylated sites on 115 proteins were identified, and the high confidence of propionylation identification was validated by parallel reaction monitoring (PRM) assay. The results show that the propionylated proteins were mostly involved in various metabolic pathways. Histones and 15 pathogenicity-related proteins were also targets for propionylation modification, suggesting their roles in epigenetic regulation and pathogenicity. A comparison of the conidial and mycelial stages revealed that most propionylated proteins and sites were growth-stage specific and independent of protein abundance. Based on the function classifications, the propionylated proteins had a similar distribution in both stages; however, some differences were also identified. Furthermore, our results show that the concentration of propionyl-CoA had a significant influence on the propionylation level. In addition to the acetylation, succinylation and propionylation identified in T. rubrum, 26 other PTMs were also found to exist in this fungus. Overall, our study provides the first global propionylation profile of a pathogenic fungus. These results would be a foundation for further research on the regulation mechanism of propionylation in T. rubrum, which will enhance our understanding of the physiological features of T. rubrum and provide some clues for the exploration of improved therapies to treat this medically important fungus.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069580345

Download citation: RISBibTeXText

PMID: 31798556


Related references

The First Whole-Cell Proteome- and Lysine-Acetylome-Based Comparison between Trichophyton rubrum Conidial and Mycelial Stages. Journal of Proteome Research 17(4): 1436-1451, 2018

Proteome-wide identification of lysine propionylation in thermophilic and mesophilic bacteria: Geobacillus kaustophilus, Thermus thermophilus, Escherichia coli, Bacillus subtilis, and Rhodothermus marinus. Extremophiles 21(2): 283-296, 2017

Lab lemco nutrient medium and urea agar for the determination of dermatophytes trichophyton mentagrophytes trichophyton rubrum trichophyton simii trichophyton verrucosum trichophyton concentricum trichophyton ferrugineum trichophyton schoenleinii trichophyton violaceum trichophyton tonsurans trichophyton sudanense. Zentralblatt fuer Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Abteilung I Originale 206(4): 550-554, 1968

Serum albumin agar used as slant for the differentiation between trichophyton rubrum and trichophyton mentagrophytes specific red pigmentation of trichophyton rubrum. Zentralblatt fuer Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Erste Abteilung Originale Reihe A Medizinische Mikrobiologie und Parasitologie 240(4): 525-528, 1978

Pigment formation by trichophyton rubrum differentiation between trichophyton rubrum and trichophyton mentagrophytes on serum albumin agar. Zentralblatt fuer Bakteriologie Parasitenkunde Infektionskrankheiten und Hygiene Erste Abteilung Originale Reihe A Medizinische Mikrobiologie und Parasitologie 237(2-3): 395-404, 1977

Proteome-wide identification of lysine succinylation in thermophilic and mesophilic bacteria. Biochimica et Biophysica Acta. Proteins and Proteomics 1865(2): 232-242, 2017

Proteome-Wide Identification of Lysine Succinylation in the Proteins of Tomato (Solanum lycopersicum). Plos one 11(2): E0147586, 2016

Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin. Journal of Proteome Research 11(2): 796-807, 2012

Urease test for the identification of trichophyton rubrum and trichophyton mentagrophytes. Hospital (Rio de Janeiro) 78(4): 213-215, 1970

Identification and characterization of propionylation at histone H3 lysine 23 in mammalian cells. Journal of Biological Chemistry 284(47): 32288-32295, 2009

The water soluble polysaccharides of dermatophytes. V. Galactomannans II from Trichophyton granulosum, Trichophyton interdigitale, Microsporum quinckeanum, Trichophyton rubrum, and Trichophyton schonleinii. Can. J. Chem 44: 2291-2297, 1966

The water-soluble polysaccharides of dermatophytes. Id. IV. Galactomannans I from Trichophyton granulosum, Trichophyton interdigitale, Microsporum quinckeanum, Trichophyton rubrum, and Trichophyton schoenleinii. Can. J. Chem 43: 30-39, 1965

Trichophyton rubrum specific immunoglobulin e in serum in patients with chronic trichophyton rubrum infection as demonstrated by crossed radioimmunoelectrophoresis. Acta Dermato-Venereologica Suppl. (120): 72-75, 1985

Genetic Ichthyosis and Trichophyton rubrum Infection in Infants: Genetische Ichthyosen und Trichophyton-rubrum-Infektionen bei kleinen Kindern. Mycoses 25(5): 281-283, 1982

Diversity of Antigenic Extracts from the Dermatophyte Trichophyton rubrum./Vergleichende Untersuchung von Antigenextrakten des Dermatophyten Trichophyton rubrum. Mycoses 30(9): 427-433, 1987