+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus



Identification of the immunodominant neutralizing regions in the spike glycoprotein of porcine deltacoronavirus



Virus Research 276: 197834



Porcine deltacoronavirus (PDCoV), is an emerging enteropathogenic coronavirus in pigs, that poses a novel threat to swine husbandry worldwide. Crucial to halting PDCoV transmission and infection is the development of effective therapies and vaccines. The spike (S) protein of coronavirus is the major target of host neutralizing antibodies, however the immunodominant neutralizing region in the S protein of PDCoV has not been defined. Here, three truncations of the PDCoV S protein were generated, the N-terminal domain of the S1 subunit (NTD, amino acids (aa) 50-286), the C-terminal domain of the S1 subunit (CTD, aa 278-616), and S2 subunit (aa 601-1087). The proteins were expressed using an E. coli expression system. Polyclonal antisera against the three recombinant proteins were produced in rabbits and mice. All three antisera were able to inhibit PDCoV infection in vitro, as determined by virus neutralization assay, fluorescent focus neutralization assay, and plaque-reduction neutralization. The CTD-specific antisera had the most potent PDCoV-neutralizing effect, indicating that the CTD region may contain the major neutralizing epitope(s) in the PDCoV S protein. Based on these findings, CTD may be a promising target for development of an effective vaccine against PDCoV infection in pigs.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069596144

Download citation: RISBibTeXText

PMID: 31816342


Related references

Identification of two neutralizing regions on the severe acute respiratory syndrome coronavirus spike glycoprotein produced from the mammalian expression system. Journal of Virology 79(3): 1906-1910, 2005

The S2 glycoprotein subunit of porcine epidemic diarrhea virus contains immunodominant neutralizing epitopes. Virology 509: 185-194, 2017

Identification of a peptide derived from the heptad repeat 2 region of the porcine epidemic diarrhea virus (PEDV) spike glycoprotein that is capable of suppressing PEDV entry and inducing neutralizing antibodies. Antiviral Research 150: 1-8, 2018

Identification of an immunodominant epitope in the C terminus of glycoprotein 5 of porcine reproductive and respiratory syndrome virus. Journal of General Virology 82(Pt 5): 995-999, 2001

Identification of an immunodominant linear neutralization domain on the S2 portion of the murine coronavirus spike glycoprotein and evidence that it forms part of a complex tridimensional structure. Journal of Virology 67(3): 1185-1194, 1993

Detection and spike gene characterization in porcine deltacoronavirus in China during 2016-2018. Infection, Genetics and Evolution 2019, 2019

Cryo-Electron Microscopy Structure of Porcine Deltacoronavirus Spike Protein in the Prefusion State. Journal of Virology 92(4):, 2018

Glycan Shield and Fusion Activation of a Deltacoronavirus Spike Glycoprotein Fine-Tuned for Enteric Infections. Journal of Virology 92(4):, 2018

Identification of immunodominant T-cell epitopes present in glycoprotein 5 of the North American genotype of porcine reproductive and respiratory syndrome virus. Vaccine 26(36): 4747-4753, 2008

Identification of an antigenic determinant on the S2 domain of the severe acute respiratory syndrome coronavirus spike glycoprotein capable of inducing neutralizing antibodies. Journal of Virology 78(13): 6938-6945, 2004

Identification of novel functional regions within the spike glycoprotein of MHV-A59 based on a bioinformatics approach. Virus Research 189: 177-188, 2014

Screening and identification of B cell antigenic Epitopes of porcine epidemic diarrhea virus spike glycoprotein by phage display. Progress in Biochemistry and Biophysics 34(9): 971-977, 2007

Identification of a novel linear B-cell epitope within the collagenase equivalent domain of porcine epidemic diarrhea virus spike glycoprotein. Virus Research 266: 34-42, 2019

Identification of multiple potent neutralizing and non-neutralizing antibodies against Epstein-Barr virus gp350 protein with potential for clinical application and as reagents for mapping immunodominant epitopes. Virology 536: 1-15, 2019

Identification and subcellular localization of porcine deltacoronavirus accessory protein NS6. Virology 499: 170-177, 2016