+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in Saccharomyces cerevisiae



Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in Saccharomyces cerevisiae



G3 2019



Zinc is essential for almost all living organisms, since it serves as a crucial cofactor for transcription factors and enzymes. However, it is toxic to cell growth when present in excess. The present work aims to investigate the toxicity mechanisms induced by zinc stress in yeast cells. To this end, 108 yeast single-gene deletion mutants were identified sensitive to 6 mM ZnCl2 through a genome-wide screen. These genes were predominantly related to the biological processes of vacuolar acidification and transport, polyphosphate metabolic process, cytosolic transport, the process utilizing autophagic mechanism. A result from the measurement of intracellular zinc content showed that 64 mutants accumulated higher intracellular zinc under zinc stress than the wild-type cells. We further measured the intracellular ROS (reactive oxygen species) levels of 108 zinc-sensitive mutants treated with 3 mM ZnCl2 We showed that the intracellular ROS levels in 51 mutants were increased by high zinc stress, suggesting their possible involvement in regulating ROS homeostasis in response to high zinc. The results also revealed that excess zinc could generate oxidative damage and then activate the expression of several antioxidant defenses genes. Taken together, the data obtained indicated that excess zinc toxicity might be mainly due to the high intracellular zinc levels and ROS levels induced by zinc stress in yeast cells. Our current findings would provide a basis to understand the molecular mechanisms of zinc toxicity in yeast cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 069613931

Download citation: RISBibTeXText

PMID: 31836620


Related references

High density array screening to identify the genetic requirements for transition metal tolerance in Saccharomyces cerevisiae. Metallomics 3(2): 195-205, 2011

Improvement of acetic acid tolerance of Saccharomyces cerevisiae using a zinc-finger-based artificial transcription factor and identification of novel genes involved in acetic acid tolerance. Applied Microbiology and Biotechnology 99(5): 2441-2449, 2015

Identification by phenotypic and genetic approaches of an indigenous Saccharomyces cerevisiae wine strain with high desiccation tolerance. Yeast 34(10): 417-426, 2017

Expression of ZRC1 coding for suppressor of zinc toxicity is induced by zinc-starvation stress in Zap1-dependent fashion in Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 276(3): 879-884, 2000

Identification and genetic analysis of Saccharomyces pombe cDNA that suppress deletion of IRA1 in Saccharomyces cerevisiae. Gene 29(1): 147-152, 1993

Calcium dependence of eugenol tolerance and toxicity in Saccharomyces cerevisiae. Plos one 9(7): E102712, 2014

Zinc uptake and toxicity in the yeasts sporobolomyces roseus and saccharomyces cerevisiae. Journal of General Microbiology 129(11): 3421-3426, 1983

On the molecular relationships between high-zinc tolerance and aconitase (Aco1) in Saccharomyces cerevisiae. Metallomics 6(3): 634-645, 2014

Yap1 mediates tolerance to cobalt toxicity in the yeast Saccharomyces cerevisiae. Biochimica et Biophysica Acta 1840(6): 1977-1986, 2014

Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae. Nucleic Acids Research 31(3): 826-832, 2003

Zinc oxide and silver nanoparticles toxicity in the baker's yeast, Saccharomyces cerevisiae. Plos one 13(3): E0193111, 2018

Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae. Molecular Microbiology 62(1): 263-277, 2006

Identification of chaperones in freeze tolerance in Saccharomyces cerevisiae. Journal of Microbiology 50(5): 882-887, 2012

Wild type RTA and less toxic variants have distinct requirements for Png1 for their depurination activity and toxicity in Saccharomyces cerevisiae. Plos one 9(12): E113719, 2014

The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity. Febs Letters 579(25): 5697-5703, 2005