Section 71
Chapter 70,108

Colorimetric strategy for ascorbic acid detection based on the oxidase-like activity of silver nanoparticle single-walled carbon nanotube composites

Li, F.; Yu, Y.; Xiao, F.; Liang, H.; Liu, C.; Fan, P.; Yang, S.

Luminescence the journal of biological and chemical luminescence 35(7): 1084-1091


ISSN/ISBN: 1522-7235
PMID: 32390340
DOI: 10.1002/bio.3820
Accession: 070107655

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A colorimetric assay for the determination and quantification of ascorbic acid (AA) is presented using silver nanoparticle (AgNP) single-walled carbon nanotube (AgNP/SWCNT) nanocomposites prepared using a microwave-assisted method. The AgNP/SWCNT nanocomposites possessed oxidase-like properties toward 3,3',5,5'-tetramethylbenzidine (TMB) and could catalyze the oxidation of TMB to form a blue oxidation product (λmax = 652 nm) in the absence of H2 O2 . AA can specifically inhibit the oxidation of TMB, resulting in a decline of the absorbance value and blue colour fading. As such, amounts of AA can be assessed easily by the unaided eye and quantitatively using an ultraviolet-visible light spectrophotometer. Under the optimal reaction conditions, this strategy showed a good linearity ranging from 0.4 μM to 5.0 μM for AA detection, and the limit of detection was 130 nM. This assay was also applied for AA measurement in vitamin C tablets and juice samples that yielded satisfactory results.

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