Section 71
Chapter 70,139

Detection of transgenic Dna in tilapias ( Oreochromis niloticus , Gift strain) fed genetically modified soybeans (Roundup Ready)

Ran, T.; Mei, L.; Lei, W.; Aihua, L.; Heru, H.R.; Jie, S.

Aquaculture Research 40(12): 1350-1357


ISSN/ISBN: 1355-557X
DOI: 10.1111/j.1365-2109.2009.02187.x
Accession: 070138176

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We used nested-polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10−10gμL−1 Dna solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous Dna segments. We also compared genetically modified (GM) soybean with non-Gm soybean diets in feeding tilapias (Oreochromis niloticus, Gift strain) and examined the residual fragments (254bp) of Gm soybeans. Tilapias receiving Gm soybean diets had Dna fragments in different tissues and organs, indicating that exogenous Gm genes were absorbed systemically and not completely degraded by the tilapia's alimentary canal.

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