Extracellular Enzyme System Utilized by the Fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the Breakdown of Cellulose : 2. Activities of the Five Endo-1,4--glucanases towards Carboxymethylcellulose
Almin, K.E.; Eriksson, K.E.; Pettersson, B.
FEBS Journal 51(1): 207-211
ISSN/ISBN: 1742-464X DOI: 10.1111/j.1432-1033.1975.tb03920.x
A complementary discussion is given of an earlier presented theory for viscometric determination of enzymic activity towards polymer substrates. Five different, purified endo-1,4-beta-glucanases from the fungus Sporotrichum pulverulentum have been characterised with respect to their molecular activities(Am) and Michaelis-Menten constants (Km). The errors in the Am and Km values are discussed. The Am value seems to be more valuable as a distinguishing parameter than the Km value.