Purification and crystallization of hemorrhagic factor, Hr2b, from the venom of Trimeresurus flavoviridis (habu)

Yonaha, K.; Iha, M.; Tomihara, Y.; Nozaki, M.; Yamakawa, M.; Kamura, T.; Toyama, S.

Toxicon 26(12): 1205-1208


ISSN/ISBN: 0041-0101
DOI: 10.1016/0041-0101(88)90305-4
Accession: 070436699

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The hemorrhagic factor, HR2b, was purified from the venom of Trimeresurus flavoviridis (habu) by a combination of gel filtration, cation exchange column chromatography and high performance liquid chromatography. The purified HR2b was homogeneous by the criteria of ultracentrifugation and SDS-disc electrophoresis. The mol. wt of HR2b was 18,000 and 18,500 by gel filtration on Sephadex G-50 and by SDS-disc electrophoresis, respectively, indicating a monomer structure for the hemorrhagic factor. Crystals of HR2b, taking the form of thin plates, were obtained in the presence of ammonium sulfate.