+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Efficiency of microspore embryogenesis in Brassica rapa using different genotypes and culture conditions

Efficiency of microspore embryogenesis in Brassica rapa using different genotypes and culture conditions

Journal of Plant Biotechnology 41(3): 116-122

ISSN/ISBN: 1229-2818

Total of fifty accessions of Brassica rapa withvarious morphological characteristics were used for productionof double haploid plants though microspore culture inBrassica rapa. Among them, only 30 accessions inducedembryos from microspores. The highest efficiency of embryoinduction of 1.194 per bud was obtained from IT135449 ofturnip type, while 3 accessions of sarson (winter oil) type didnot generate embryo. The effect of heat shock periods forembryogenesis was also investigated with 4 accessions(IT135449; Turnip type, IT199710; Chinese cabbage type,IT212886; Pak choi type, IT218043; Summer oil type). Thehigh productions of embryos were observed in IT135449,IT199710 and IT212886 when microspores were pre-culturedto 32˚C for 2 days. In IT218043, high embryogenesis wasobserved at the 3 days of heat shock treatment. The optimalcondition of shoot regeneration for IT199710 was observedin MS medium supplemented with NAA 0.5 mg·L-1 andBAP 1 mg·L-1. In contrast, the IT135449 and IT212886 wereobserved high regeneration frequency in MS medium withoutplant growth regulators. All the plantlets regenerated frommicrospore-derived embryos have been successfully transplantedto soil, and bud self-pollinated seeds were produced fromdoubled haploid plants. This indicated that double-haploidgenotype was likely generated naturally during embryogenesisprocess.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 070461299

Download citation: RISBibTeXText

Related references

Effects of genotypes and culture conditions on microspore embryogenesis and plant regeneration in several subspecies of Brassica rapa L. Plant Biotechnology Reports 6(4): 297-304, 2012

Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L. Plants 9(2), 2020

High efficiency microspore culture system construction of Brassica napus I Factors involved in microspore embryogenesis. Jiangsu Journal Of Agricultural Sciences: 6, 792-796, 2008

Effect of sucrose concentration on Brassica rapa rapid-cycling microspore culture embryogenesis. Biotechnological approaches for utilisation of gametic cells COST 824: Final Meeting, Bled, Slovenia, 1-5 July 2000: 119-124, 2001

The effect of culture shaking on microspore embryogenesis and embryonic development in Pakchoi Brassica rapa L ssp chinensis. Scientia Horticulturae 152: 70-73, 2013

Embryogenesis and doubling mechanism of isolated microspore culture in Chinese cabbage (Brassica rapa ssp. pekinensis). Acta Horticulturae Sinica 33(5): 974-978, 2006

Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture. Plant Cell Reports 13(8): 447-450, 1994

Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line. Plant Cell Reports 14(9): 580-584, 1995

Mapping of loci affecting the cultural efficiency of microspore culture of Brassica rapa L. syn. campestris L. using DNA polymorphism. Breeding Science 49(3): 187-192, 1999

Influence of culture duration and conditions on embryogenesis of isolated microspore culture in cabbage (Brassica oleraceae L. var. capitata). Journal of Plant Biotechnology 44(1): 27-34, 2017

PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea. Plant cell tissue and organ culture 86(2): 201-210, 2006

Effects of the antiauxin PCIB on microspore embryogenesis and plant regeneration in Brassica rapa. Scientia Horticulturae 130(1): 32-37, 2011

Effects of the antiauxin PCIB on microspore embryogenesis and plant regeneration in Brassica rapa. Scientia Horticulturae 130(1): 0, 2011

Effects of brassinolide on microspore embryogenesis and plantlet regeneration in pakchoi (Brassica rapa var. multiceps). Scientia Horticulturae 252: 354-362, 2019

Effect of thidiazuron on microspore embryogenesis and plantlet regeneration in Chinese flowering cabbage (Brassica rapa. var. parachinenis). Plant Breeding 138(6): 916-924, 2019