A Rapid Identification of Korean Ginseng Cultivar, Cheonryang, using Specific DNA Markers
Jo, I.-H.; Chang, K.Y.; Soo, N.B.; Hyun, D.Y.; KIM, D.O.N.G. H.W.I.; Bang, K.; ๊น์ฅ์ฑ; ์ด์นํธ; ์์ง์; ๋ฌธ์ง์; ๊น๊ธฐํ
Korean Journal of Medicinal Crop Science 22(6): 429-434
2014
ISSN/ISBN: 1225-9306 Accession: 070461620
This study describes the efficient method for the discrimination of 'Cheonryang' in Panax ginseng Meyerusing a STS primer. A total of 208 STS primers were applied to polymerase chain reaction (PCR) amplification for discriminatingKorean ginseng cultivars. Co-dominant polymorphic band patterns were generated with two primers, MFGp 0019,MFGp 0248, and successful identification of 'Cheonryang' was achieved from out of 11 Korean ginseng cultivars. Two differentsizes of DNA band patterns were detected with MFGp 0019 primer. Ten Korean ginseng cultivars shared the samesize of amplified DNAs (389 bp), but 'Cheonryang' showed a different size. Thus 'Cheonryang' can be efficiently distinguishedfrom the other ten ginseng cultivars by using the MFGp 0019 primer. In the case of MFGp 0248, two different sizesof DNA band patterns were detected in the eleven ginseng cultivars. Same sized amplified DNA bands (307 bp) were shownin five cultivars (Chunpoong, Gopoong, Kumpoong, Cheongsun, Sunhyang) and 254 bp sized DNA bands were identified inthe other 6 cultivars (Yunpoong, Sunpoong, Sunun, Sunone, Cheonryang, K-1). In conclusion, the two STS primers, MFGp0019, and MFGp 0248, provide a rapid and reliable method for the specific identification of 'Cheonryang' cultivar from alarge number of samples.