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Detection of Sugar Cane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey


Detection of Sugar Cane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey



Journal of Apiculture 33(3): 221-226



ISSN/ISBN: 1225-0252

Sugar cane-specific gene could be successfully amplified with DNAs isolated from sugar or sugarhoney using Saccharum officinarum-specific Ultra-Rapid or conventional PCR. Specificity of PCR products from sugar or sugar-honey was verified by nested PCR and DNA sequencing. This PCR could be applied to a quantitative analysis for honey-evaluation. In our knowledge, it is first report that sugar cane-specific sequence could be detected from sugar-honey or sugar itself, and that sugar-honey could be evaluated by genetic examination.

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Accession: 070468280

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