Detection of Sugar Cane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey
Detection of Sugar Cane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey
Hee, K.B.; somin, K.; Kim, M.; Min, K.J.; Tai, T.A.; Yoon, B.-S.
Journal of Apiculture 33(3): 221-226
2018
ISSN/ISBN: 1225-0252
Sugar cane-specific gene could be successfully amplified with DNAs isolated from sugar or sugarhoney using Saccharum officinarum-specific Ultra-Rapid or conventional PCR. Specificity of PCR products from sugar or sugar-honey was verified by nested PCR and DNA sequencing. This PCR could be applied to a quantitative analysis for honey-evaluation. In our knowledge, it is first report that sugar cane-specific sequence could be detected from sugar-honey or sugar itself, and that sugar-honey could be evaluated by genetic examination.