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Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Subbaiyan, B.; Thangapandian, V.

Botanica Pacifica: a journal of plant science and conservation 6(2): 35-42

2017


ISSN/ISBN: 2226-4701
Accession: 070505149

An efficient protocol of callus induction, shoot multiplication and plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa has been elaborated. The highest percentage (90.66) of callus induction was observed when cotyledonary explants cultured on MS medium having 0.5 mg/L 2,4-D in combination with 0.05 mg/L Kn followed by 86.25 % callus induction from leaf explant at 0.5 mg/l BAP in combination with 0.05 mg/l Kn. Shoot multiplication was observed, cotyledon derived callus cultured on 1.0 mg/L BAP in combination with 0.10 mg/L NAA could induce number of shoots (4.71) and their lengths (6.13 cm). Cotyledonary callus derived microshoots gave the highest rooting percentage (92.26 %) root numbers (13.14) and their lengths (8.87 cm) were observed on half-strength MS medium fortified with 0.4 mg/L IBA. In vitro derived plantlets were hardened in the greenhouse and successfully established into the soil.

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