Effect of beta-mercaptoethanol and buffalo follicular fluid on fertilization and subsequent embryonic development of water buffalo (Bubalus bubalis) oocytes derived from in vitro maturation
Shang, J. H.; Huang, Y. J.; Zhang, X. F.; Huang, F. X.; Qin, J.
Italian Journal of Animal Science 6: 751-754
This study investigated effects of beta-mercaptoethanol (beta-ME) and buffalo follicular fluid (buFF) on IVF and embryonic development of water buffalo oocytes derived from IVM. In Experiment 1, beta-ME (50 mu M and 100 mu M, treatments) was added in the media during IVM and IVC but not IVF. A highly significant difference was obtained in rate of blastocyst (9.26% Vs 26.72% or 27.04%, P<0.01), whereas no significant differences in rates of cleavage and hatched blastocyst between control group (0 mu M) and treatment groups (50 mu M and 100 mu M). Also, no significant differences (P>0.05) were observed in rates of cleavage, blastocyst and hatched blastocyst between two treatment groups, respectively. In Experiment 2, comparison with 10% FBS, significant and highly significant differences were recorded in rate of blastocyst in 5% FBS + 5% buFF (17.16% vs 27.64%, P<0.05)and 10% buFF (17.16% vs 32.98%, P<0.01), respectively. These results indicate that supplementation of beta-ME in IVM and IVC and replacement of FBS with buFF in IVM media improve remarkably the development of water buffalo embryos derived from IVM-IVF to the blastocyst stage.