Section 71
Chapter 70,611

Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima)

Ndjiondjop, M.N.; Semagn, K.; Sie, M.; Cissoko, M.; Fatondji, B.; Jones, M.

African Journal of Biotechnology 7(23): 4219-4229


ISSN/ISBN: 1684-5315
Accession: 070610948

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Several lowland NERICAs (New Rice for Africa) were derived from crosses between IR64 (an Oryza sativa subsp. indica variety) and Tog5681 (an Oryza glaberrima variety) that possess useful traits adapted to lowland conditions in West Africa. The proportion of parental genomic contribution and extent of genetic differences among these sister lines is unknown at the molecular level. The objectives in this study were therefore to determine, with 60 SSR markers that cover 1162 cM of the rice genome, the frequency and magnitude of deviations from the expected parental contributions among 21 BC(2)F(10), 17 BC(3) F(8) and 10 BC(4)F(8) lines and determine patterns of their genetic relationships. The estimated average O. glaberrima genome coverage was 7.2% (83.5 cM) at BC(2)F(10), 8.5% (99.3 cM) at BC(3)F(8) and 8.1% (93.8 cM) at BC(4)F(8) lines. The O. sativa parent accounted for 73.2% (851.3 cM) at BC(2)F(10), 82.6% (959.5 cM) at BC(3)F(8) and 78.2% (908.6 cM) at BC(3)F(8). Non-parental alleles were detected at all 3 backcross generations but the frequency of such alleles at BC(2) (8.8%) was twice that of BC(3)F(8) (3.4%) and nine times that of BC(4)F(8) (0.9%). Both cluster and principal component analyses revealed two major groups irrespective of the level of backcross generations and the proportion of parental genome contribution.

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