Fingerprinting of Corynebacterium Pseudotuberculosisstrains by Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Techniques
Nowicki, M.; Kaba, J.; Mosiej, E.; Lutynska, A.; Augustynowicz, E.; Rzewuska, M.; Binek, M.; Szalus-Jordanow, O.; Stefanska, I.; Frymus, T.
Bulletin of the Veterinary Institute in Pulawy 55(3): 403-409
ISSN/ISBN: 0042-4870 Accession: 070660606
In the study, phenotypic and genotypic properties of 42 Corynebacterium pseudotuberculosis strains isolated from 16 goat flocks were determined by random amplified polymorphic DNA and amplified fragment length polymorphism techniques. Several sets of RAPD primers and AFLP protocols were experimentally evaluated for potential of generating amplification profiles of the best discriminatory power. The overall similarity level of profiles by cluster analysis generated with RAPD3 (64%) set was higher than obtained for both RAPD 2 (70%) and AFLP (88%). The results confirmed usefulness of both AFLP and RAPD methods to discriminate strains of C. pseudotuberculosis, even at the flocks' level, and confirmed a single genotype as the most prevalent in goats coming from flocks in Poland.