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Plasma Progesterone Radioimmunoassay as a Tool to Confirm Ovarian Cyclicity of Recipients Following Nonsurgical Transfer of Cloned Swamp Buffalo (Bubalus bubalis Lin.) Embryo

Plasma Progesterone Radioimmunoassay as a Tool to Confirm Ovarian Cyclicity of Recipients Following Nonsurgical Transfer of Cloned Swamp Buffalo (Bubalus bubalis Lin.) Embryo

Buffalo Bulletin 32: 537-540

ISSN/ISBN: 0125-6726

The purpose of this study was the used of plasma progesterone (P4) profiles for assessing cyclic ovarian activity in ten swamp buffalo (Bubalus bubalis Lin.) recipients. Blood sampling were collected 10 days interval and at the day of transferring embryo from jugular vein into heparinized tubes. Plasma was separated and stored at -20 degrees C until P4 was determined by radioimmunoassay (RIA). The ovary containing a palpable functional corpus luteum (CL) of recipients was transferred non-surgically with 1-3 cloned swamp buffalo embryos at various stage. Plasma P4 levels showed on the day of cloned embryo were transferred, the recipients were in follicular phase, early-luteal phase, mid-luteal phase, late-luteal phase, anoestrous and ovulation while mean +/- SD plasma P4 were 0.37(n=1), 0.75 +/- 0.06 (n=3), 1.88 +/- 0.54(n=11), 0.78 +/- 0.19(n=4), 0.04 (n=1) and 0.21 +/- 0.04 (n=3) ng/ml, respectively. And the accuracy of rectal palpation was 78.0%. In the present study pregnant recipients with plasma P4 concentration >0.52 ng/ml and maintained beyond 80 days were found when transferred of morula, blastocyst and blastocyst + hatched blastocyst to animals which were in late-luteal phase, mid-luteal phase, early- and mid-luteal phase, respectively. One recipient, which received two blastocyst at mid-luteal phase, gave birth at Day 326 to one calf on 25 October, 2011. In conclusions, plasma P4 RIA could use as a tool to confirm ovarian cyclicity of recipients as follows; 1) evaluation stage of oestrous cycle; and 2) following up period of gestation. Therefore, these will improve successful pregnancies after embryo transfer.

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Accession: 070712429

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