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Production of beta-ecdysone in in vitro-cultured Brazilian ginseng, Pfaffia glomerata (Spreng.) Pedersen

Production of beta-ecdysone in in vitro-cultured Brazilian ginseng, Pfaffia glomerata (Spreng.) Pedersen

Journal of Horticultural Science and Biotechnology 90(2): 109-114

ISSN/ISBN: 1462-0316

DOI: 10.1080/14620316.2015.11513160

In Brazil, several pharmaceutical industries produce phytomedicines from the roots of Pfaffia glomerata (Spreng.) Pedersen. In this work, callus cultures of P. glomerata were established in order to study whether in vitro-cultured tissue could produce beta-Ecdysone. To induce calli, nodal segments were cultured on Murashige and Skoog (MS) medium with various levels of 6-benzylaminopurine (BAP; 1.0, 5.0, or 10.0 mu M), alpha-naphthaleneacetic acid (NAA; 0.5, 1.0, 5.0, or 10.0 mu M) or 2,4-dichlorophenoxyacetic (2,4-D; 0.5, 1.0, 5.0, or 10.0 mu M). beta-Ecdysone concentrations [on a (w/w) dry weight (DW) basis] in in vitro-cultured and field-grown tissues were measured by high performance liquid chromatography. The level of beta-ecdysone was related to the friability and shoot regeneration capacity of the different calli. Organogenic, friable calli grown on media supplemented with 10.0 mu M BAP, plus either 5.0 mu M NAA or 0.5 mu M 2,4-D, resulted in the highest beta-ecdysone concentrations [0.282% (w/w) and 0.211% (w/w), respectively]. In contrast, the beta-ecdysone concentrations in field-grown plants were 0.194% (w/w) in roots and 0.333% (w/w) in the aerial parts. Low levels of beta-ecdysone [0.007% (w/w)] were detected in plantlets cultured on hormone-free MS medium, indicating the importance of culture conditions for the in vitro production of beta-ecdysone. This is the first report on the presence of ecdysteroids in calli of P. glomerata.

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