Bioactive metabolites from the ruminal bacterium Enterobacter amnigenus ZIH
Bioactive metabolites from the ruminal bacterium Enterobacter amnigenus ZIH
El Euch, I.Z.; Shaaban, K.A.; Riaz, N.; Bahi, M.; Shaaban, M.
Indian Journal of Natural Products and Resources 9(3): 222-228
2018
ISSN/ISBN: 0976-0504
Bioassay-guided fractionation and chromatographic purification of the crude extract of ruminal bacterium Enterobacter amnigenus ZIH (isolated from cattle) produce one new microbial product: butyl-alpha-D-glucopyranoside together with eleven known compounds including (S)-brevinic acid, 3-(hydroxyacetyl)-indole, N-beta-acetyltryptamine, tyrosol, phenol, tryptophol, indole-3-lactic acid, uracil, adenine and two diketopiperazines cyclo-(Phe, Pro) and cyclo-(Leu, Pro). The complete NMR assignments of butyl-alpha-D-glucopyranoside was done - using different spectroscopic (H-1, C-13, H-1-H-1 COSY, HMQC, and HMBC) and spectrometric methods (ESI-MS, HRESI-MS). Compounds 1a and 2 reported for the first time from a natural source. The bacterial extract exhibited high cytotoxicity against brine shrimp and moderate antimicrobial activity against a diverse set of pathogenic bacteria strains.