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Embryogenic callus induction and Agrobacterium -mediated genetic transformation of 'Shine Muscat' grape

Nakajima, I.; Endo, M.; Haji, T.; Moriguchi, T.; Yamamoto, T.

Plant Biotechnology 37(2): 185-194

2020


ISSN/ISBN: 1342-4580
PMID: 32821226
DOI: 10.5511/plantbiotechnology.20.0527a
Accession: 071031094

We established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar 'Shine Muscat' (Vitis labruscana). Embryogenic calli were induced using flower bud filaments from a dormant cane. Agrobacterium strain LBA4404 harboring the binary plasmid pBin19-sgfp, which contains the sgfp and nptII genes, was used to infect embryogenic calli. Infected calli were selected on 1/2 MS medium containing 5% maltose and 2% agar supplemented with 15 mg l(-1) kanamycin. Efficiency of transformation of regenerated plants reached nearly 100% as determined by PCR and Southern blot analyses. The developed method will open a new avenue for genome editing of 'Shine Muscat' and contribute to the advancement of grape breeding.

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