EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

GhCIPK6a increases salt tolerance in transgenic upland cotton by involving in ROS scavenging and MAPK signaling pathways


GhCIPK6a increases salt tolerance in transgenic upland cotton by involving in ROS scavenging and MAPK signaling pathways



BMC Plant Biology 20(1): 421



ISSN/ISBN: 1471-2229

PMID: 32928106

DOI: 10.1186/s12870-020-02548-4

Background Salt stress is one of the most damaging abiotic stresses in production of Upland cotton (Gossypium hirsutum). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality. Results Our previous study verified aGhCIPK6agene response to salt stress inG. hirsutum. The homologs ofGhCIPK6awere analyzed in A(2)(G. arboreum), D-5(G.raimondii), and AD(1)(G. hirsutum) genomes. GhCIPK6a localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6a and GhCBL8-GhCIPK6a complexes localized to the nucleus and cytomembrane. Overexpression ofGhCIPK6aenhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds fromGhCIPK6aoverexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages ofGhCIPK6aoverexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing ofGhCIPK6ain cotton increased lint percentage, and fiber length uniformity under salt stress. Conclusions We verified the function ofGhCIPK6aby transformation and RNA-seq analysis.GhCIPK6aoverexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore,GhCIPK6ahas the potential for cotton breeding to improve stress-tolerance.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 071052480

Download citation: RISBibTeXText

Related references

SnRK2 Homologs in Gossypium and GhSnRK2.6 Improved Salt Tolerance in Transgenic Upland Cotton and Arabidopsis. Plant Molecular Biology Reporter 35(4): 442-456, 2017

A Novel G-Protein-Coupled Receptors Gene from Upland Cotton Enhances Salt Stress Tolerance in Transgenic Arabidopsis. Genes 9(4), 2018

GhSOS1, a plasma membrane Na+/H+ antiporter gene from upland cotton, enhances salt tolerance in transgenic Arabidopsis thaliana. Plos one 12(7): E0181450, 2017

A novel cotton WRKY gene, GhWRKY6-like, improves salt tolerance by activating the ABA signaling pathway and scavenging of reactive oxygen species. Physiologia Plantarum 162(4): 439-454, 2018

Mechanisms and Signaling Pathways of Salt Tolerance in Crops: Understanding from the Transgenic Plants. Tropical Plant Biology 13(4): 297-320, 2020

Analyzing serial cDNA libraries revealed reactive oxygen species and gibberellins signaling pathways in the salt response of Upland cotton (Gossypium hirsutum L.). Plant Cell Reports 34(6): 1005-1023, 2015

Transcriptome analysis reveals that distinct metabolic pathways operate in salt-tolerant and salt-sensitive upland cotton varieties subjected to salinity stress. Plant Science: An International Journal of Experimental Plant Biology 238: 33-45, 2015

Chondroprotective effects of glucosamine involving the p38 MAPK and Akt signaling pathways. Rheumatology International 28(10): 1009-1016, 2008

Co-overexpression ofAVP1andAtNHX1in Cotton Further Improves Drought and Salt Tolerance in Transgenic Cotton Plants. Plant Molecular Biology Reporter 33(2): 167-177, 2015

Genetic Variation in Salt Tolerance during Seed Germination in a Backcross Inbred Line Population and Advanced Breeding Lines Derived from Upland Cotton x Pima Cotton. Crop Science 53(5): 1974-1982, 2013

Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton. Plant Molecular Biology 87(1-2): 47-67, 2015

Co-expression of AtNHX1 and TsVP improves the salt tolerance of transgenic cotton and increases seed cotton yield in a saline field. Molecular Breeding 38(2): 19, 2018

Expression of mtlD gene in transgenic upland rice leads to enhanced salt-tolerance. Journal of China Agricultural University 9(6): 38-43, 2004

The studies on salt-tolerance of transgenic mtlD and BADH upland rice at seedling stage. Southwest China Journal of Agricultural Sciences 18(5): 621-624, 2005

RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in Upland cotton. Plant Science: An International Journal of Experimental Plant Biology 271: 52-61, 2018