The Comparison of Iron Deficiency Anemia Rat Treated with Hydroxypropyl Chitosan Ferrous Ion Complex and Ferrous Sulfate
Huang, M.; Ye, C.; Liu, L.-L.; Tian, C.; Yan, W.-J.; Ye, Z.-L.
Zhongguo Shi Yan Xue Ye Xue Za Zhi 29(3): 853-859
To observe and compare the therapeutic effects of hydroxypropyl chitosan ferrous ion complex solution and ferrous sulfate solution in iron deficiency anemia rats and their effects on gastric mucosa. Seven rats were randomly selected from thirty five SPF grade SD rats as control group, and were fed with normal diet, distilled water (E). The rest of SD rats were fed with low iron feed and distilled water plus continuous tail vein bloodletting to establish the iron deficiency anemia model. After the model was established successfully, the rats were randomly divided into four groups: blank control group (A), iron deficiency anemia control group (B), ferrous sulfate group (C), hydroxypropyl chitosan ferrous ion complex (HPCTS-Fe2+) group (D). Group A was killed and the gastric tissue was taken to make the pathological section. Group E was fed with normal feed and distilled water continually. Group B, C and D were fed with low iron feed and distilled water. Moreover, Group C and group D were treated with ferrous sulfate solution and HPCTS-Fe2+ solution respectively to made the blood recover treatment (4 mg / kg of iron each time, twice a day). During the treatment period, Ret%, SF and blood routine were checked regularly. After 6 weeks of treatment, the rats were killed, and the gastric tissue was taken to make the pathological section, and the condition of gastric mucosa was observed by the light microscope. After modeling, except the normal control group, the hair color of the rats in the four groups showed dark yellow and the belly of the toes became white gradually. HGB, HCT, Ret%, MCV, MCH, MCHC and SF decreased significantly (P < 0.05). After treatment, the rats with dark yellow hair in group C and D were improved, and the toe abdomen turned pink gradually. RBC, HGB, HCT, Ret%, MCV, MCH, MCHC and SF in rats in group C and D increased, which were higher than those in group B (P < 0.05). The HGB of the rats in group D was higher than that of group C in day 28th during treatment and the Ret% was higher than that in group C at day 10th (P<0.05).After treatment, the liver and spleen of the rats in group C and D were lighter than those in group B (P<0.05).The gastric mucosa in group A, B, D and E was not damaged obviously, while it was slightly irritated and damaged in group C. Hydroxypropyl chitosan ferrous complex solution can improve the hemoglobin level of SD rats with iron deficiency anemia, which is stronger than ferrous sulfate solution and shows no damage to gastric mucosa. 羟丙基壳聚糖亚铁离子配合物和硫酸亚铁对缺铁性贫血大鼠治疗的对比研究. 观察比较羟丙基壳聚糖亚铁离子配合物和硫酸亚铁对缺铁性贫血大鼠的治疗效果及对其胃黏膜的影响. 从35只SPF级别SD大鼠中随机选取出7只设为正常对照组（E），给予普通饲料和蒸馏水喂养。其余SD大鼠采用低铁饲料和蒸馏水喂养，加尾静脉连续放血法进行缺铁性贫血建模。建模成功后，随机分成4组，分别为胃黏膜空白对照组（A）、缺铁性贫血对照组（B）、硫酸亚铁组（C）、羟丙基壳聚糖亚铁离子配合物（HPCTS-Fe2+）组（D），A组处死并取胃组织制作病理切片，E组继续予普通饲料和蒸馏水喂养，B、C、D组继续予低铁饲料和蒸馏水，另外C组和D组分别予硫酸亚铁溶液和HPCTS-Fe2+溶液（铁均为每次4 mg/kg，每天2次）进行贫血恢复治疗。所有大鼠在治疗期间，定期检查Ret%、SF和血常规等指标。治疗6周后处死大鼠，取其胃组织制作胃病理切片，在光学显微镜下观察胃黏膜的情况. 造模前后对比，除正常对照组外4组大鼠毛色暗黄，趾腹逐渐变白，HGB、Hct、Ret%、MCV、MCH、MCHC、SF明显降低（P<0.05）。治疗前后对比，C、D组经治疗后毛色暗黄改善，趾腹逐渐变粉色，RBC、HGB、Hct、Ret%、MCV、MCH、MCHC、SF均明显升高，且高于B组（P<0.05）。D组HGB在治疗d 28时高于C组，Ret%在治疗d10、d14时高于C组（P<0.05）。治疗后C、D两组肝脾重量较B组减轻（P<0.05）。胃组织病理对比，A、B、D、E组大鼠胃黏膜未见明显破坏，C组胃黏膜有轻微刺激和损伤. 羟丙基壳聚糖亚铁离子配合物溶液能提高缺铁性贫血SD大鼠的血红蛋白水平，作用比硫酸亚铁溶液强，对胃黏膜无损害.