Studies on the antigenic structure of Histoplasma farciminosum. II. Serological activity in the CF test of various antigenic fractions
Polskie Archiwum Weterynaryjne 16(Fasc. 3): 415-431
ISSN/ISBN: 0079-3647 Accession: 072903906
Specific antiserum against H. farciminosum was prepared by immunising rabbits. The rabbits often died with symptoms of shock, but this could be eliminated by desensitization by s/c injection of 0.1-0.2 ml of antigen 2-4 hours before i/v inoculation. Antigen prepared from mycelium dried in acetone was less toxic than native formolized antigen. Polysaccharide fractions (WW, West, Full, Ck, Cz, and R) were inactive, while protein fractions (Labz, N) and metabolic antigens were active allergens.