Top Buy Now Summary References
Home
>
Section
>
Chapter

Tissue-specific and hormonally controlled alternative promoters regulate aromatase cytochrome P450 gene expression in human adipose tissue

Mahendroo, M.S.; Mendelson, C.R.; Simpson, E.R.

Journal of Biological Chemistry 268(26): 19463-19470

1993

ISSN/ISBN: 0021-9258
PMID: 7690033
Accession: 009652345

Full-Text Article emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Summary
Estrogen biosynthesis is catalyzed by a microsomal enzyme, aromatase cytochrome P450 (P450arom; the product of the CYP19 gene). The human CYP19 gene comprises nine coding exons, II-X. Additionally, tissue-specific expression is determined by the use of tissue-specific promoters, which give rise to P450arom transcripts with unique 5'-noncoding sequences. In placenta, P450arom transcripts contain one of two 5'-untranslated exons, I.1 or I.2, while ovarian transcripts instead contain sequence consistent with the use of a promoter, PII, which is proximal to the start of translation. To characterize transcripts present in adipose tissue and adipose stromal cells (ASC) in culture, cDNA libraries were constructed by the RACE (rapid amplification of cDNA ends) procedure. Four P450arom transcripts with unique 5' termini were identified, leading to the characterization of two unique 5'-untranslated exons of the CYP19 gene, I.3 and I.4. Whereas I.3-specific sequence is expressed in adipose tissue as well as in ACS maintained under all culture conditions, I.4-specific sequence is apparently present only in breast adipose tissue, and ACS stimulated with glucocorticoids. On the other hand, PII-specific sequence is present only in cells stimulated with cAMP analogues and is absent from cells stimulated with glucocorticoids. We conclude that CYP19 gene expression in human adipose tissue likely utilizes two novel promoters and, furthermore, that alternative promoter usage in cultured ASC is a function of the hormonal environment in which the cells are maintained.

References

Means, G.D.; Kilgore, M.W.; Mahendroo, M.S.; Mendelson, C.R.; Simpson, E.R. 1991: Tissue-specific promoters regulate aromatase cytochrome P450 gene expression in human ovary and fetal tissues Molecular Endocrinology 5(12): 2005-2013
Harada, N.; Utsumi, T.; Takagi, Y. 1993: Tissue-specific expression of the human aromatase cytochrome P-450 gene by alternative use of multiple exons 1 and promoters, and switching of tissue-specific exons 1 in carcinogenesis Proceedings of the National Academy of Sciences of the United States of America 90(23): 11312-11316
Bouraïma, H.; Hanoux, V.; Mittre, H.; Féral, C.; Benhaïm, A.; Leymarie, P. 2001: Expression of the rabbit cytochrome P450 aromatase encoding gene uses alternative tissue-specific promoters European Journal of Biochemistry 268(16): 4506-4512
Simpson, E.R.; Mahendroo, M.S.; Means, G.D.; Kilgore, M.W.; Corbin, C.J.; Mendelson, C.R. 1993: Tissue-specific promoters regulate aromatase cytochrome P450 expression Journal of Steroid Biochemistry and Molecular Biology 44(4-6): 321-330
Simpson, E.R.; Mahendroo, M.S.; Means, G.D.; Kilgore, M.W.; Corbin, C.J.; Mendelson, C.R. 1993: Tissue-specific promoters regulate aromatase cytochrome P450 expression Clinical Chemistry 39(2): 317-324
Bulun, S.E.; Rosenthal, I.M.; Brodie, A.M.; Inkster, S.E.; Zeller, W.P.; DiGeorge, A.M.; Frasier, S.D.; Kilgore, M.W.; Simpson, E.R. 1993: Use of tissue-specific promoters in the regulation of aromatase cytochrome P450 gene expression in human testicular and ovarian sex cord tumors, as well as in normal fetal and adult gonads Journal of Clinical Endocrinology and Metabolism 77(6): 1616-1621
Bulun, S.E.; Rosenthal, I.M.; Brodie, A.M.; Inkster, S.E.; Zeller, W.P.; DiGeorge, A.M.; Frasier, S.D.; Kilgore, M.W.; Simpson, E.R. 1994: Use of tissue-specific promoters in the regulation of aromatase cytochrome P450 gene expression in human testicular and ovarian sex cord tumors, as well as in normal fetal and adult gonads Journal of Clinical Endocrinology and Metabolism 78(2): 1616-1621
Hinshelwood, M.M.; Mendelson, C.R. 2001: Tissue-specific expression of the human CYP19 (aromatase) gene in ovary and adipose tissue of transgenic mice Journal of Steroid Biochemistry and Molecular Biology 79(1-5): 193-201
Simpson, E.R.; Mahendroo, M.S.; Means, G.D.; Kilgore, M.W.; Corbin, C.J.; Mendelson, C.R. 1993: Tissue specific promoters regulate aromatase cytochrome P450 expression : Molecular endocrinology : current concepts in receptors, growth factors and signal transduction mechanisms National Academy of Clinical Biochemistry. Annual Meeting Symposium 39(2): 317-324
Zhao, Y.; Nichols, J.E.; Bulun, S.E.; Mendelson, C.R.; Simpson, E.R. 1995: Aromatase P450 gene expression in human adipose tissue. Role of a Jak/STAT pathway in regulation of the adipose-specific promoter Journal of Biological Chemistry 270(27): 16449-16457
Mitsui, S.; Nakamura, T.; Okui, A.; Kominami, K.; Uemura, H.; Yamaguchi, N. 2006: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11/hippostasin Febs Journal 273(16): 3678-3686
Sumi-Ichinose, C.; Hasegawa, S.; Ichinose, H.; Sawada, H.; Kobayashi, K.; Sakai, M.; Fujii, T.; Nomura, H.; Nomura, T.; Nagatsu, I. 1995: Analysis of the alternative promoters that regulate tissue-specific expression of human aromatic L-amino acid decarboxylase Journal of Neurochemistry 64(2): 514-524
Ball, H.J.; Shine, J.; Herzog, H. 1995: Multiple promoters regulate tissue-specific expression of the human NPY-Y1 receptor gene Journal of Biological Chemistry 270(45): 27272-27276
Liang, S-Hsin.; Hassett, C.; Omiecinski, C.J. 2004: Alternative promoters determine tissue-specific expression profiles of the human microsomal epoxide hydrolase gene (EPHX1) Molecular Pharmacology 67(1): 220-230
Polari, L.; Yatkin, E.; Martínez Chacón, M.G.; Ahotupa, M.; Smeds, A.; Strauss, L.; Zhang, F.; Poutanen, M.; Saarinen, N.; Mäkelä, S.I. 2015: Weight gain and inflammation regulate aromatase expression in male adipose tissue, as evidenced by reporter gene activity Molecular and Cellular Endocrinology 412: 123-130
Yang, L.V.; Heng, H.H.; Wan, J.; Southwood, C.M.; Gow, A.; Li, L. 2003: Alternative promoters and polyadenylation regulate tissue-specific expression of Hemogen isoforms during hematopoiesis and spermatogenesis Developmental Dynamics: An Official Publication of the American Association of Anatomists 228(4): 606-616
Kilgore, M.W.; Means, G.D.; Simpson, E.R. 1991: Tissue specific expression of aromatase cytochrome p 450 in the human FASEB Journal 5(4): A680
Zhang, Y.; Zhang, S.; Zhou, W.; Ye, X.; Ge, W.; Cheng, C.H.K.; Lin, H.; Zhang, W.; Zhang, L. 2012: Androgen rather than estrogen up-regulates brain-type cytochrome P450 aromatase (cyp19a1b) gene via tissue-specific promoters in the hermaphrodite teleost ricefield eel Monopterus albus Molecular and Cellular Endocrinology 350(1): 125-135
Escalante, R.; Sastre, L. 1995: Tissue-specific alternative promoters regulate the expression of the two sarco/endoplasmic reticulum Ca-ATPase isoforms from Artemia franciscana Dna and Cell Biology 14(10): 893-900
Friday, R.P.; Pietropaolo, S.L.; Profozich, J.; Trucco, M.; Pietropaolo, M. 2003: Alternative core promoters regulate tissue-specific transcription from the autoimmune diabetes-related ICA1 (ICA69) gene locus Journal of Biological Chemistry 278(2): 853-863