Top Buy Now Summary References
Home
>
Section
>
Chapter

Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating

Verstreken, P.; Koh, T-Wey.; Schulze, K.L.; Zhai, R.Grace.; Hiesinger, P.Robin.; Zhou, Y.; Mehta, S.Q.; Cao, Y.; Roos, J.; Bellen, H.J.

Neuron 40(4): 733-748

2003

ISSN/ISBN: 0896-6273
PMID: 14622578
DOI: 10.1016/s0896-6273(03)00644-5
Accession: 012607918

Full-Text Article emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Summary
We describe the isolation and characterization of Drosophila synaptojanin (synj) mutants. synj encodes a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis. We show that Synj is specifically localized to presynaptic terminals and is associated with synaptic vesicles. The electrophysiological and ultrastructural defects observed in synj mutants are strikingly similar to those found in endophilin mutants, and Synj and Endo colocalize and interact biochemically. Moreover, synj; endo double mutant synaptic terminals exhibit properties that are very similar to terminals of each single mutant, and overexpression of Endophilin can partially rescue the functional defects in partial loss-of-function synj mutants. Interestingly, Synj is mislocalized and destabilized at synapses devoid of Endophilin, suggesting that Endophilin recruits and stabilizes Synj on newly formed vesicles to promote vesicle uncoating. Our data also provide further evidence that kiss-and-run is able to maintain neurotransmitter release when synapses are not extensively challenged.

References

Song, W.; Zinsmaier, K.E. 2003: Endophilin and synaptojanin hook up to promote synaptic vesicle endocytosis Neuron 40(4): 665-667
Schuske, K.R.; Richmond, J.E.; Matthies, D.Signor.; Davis, W.S.; Runz, S.; Rube, D.A.; van der Bliek, A.M.; Jorgensen, E.M. 2003: Endophilin is required for synaptic vesicle endocytosis by localizing synaptojanin Neuron 40(4): 749-762
Pechstein, A.; Gerth, F.; Milosevic, I.; Jäpel, M.; Eichhorn-Grünig, M.; Vorontsova, O.; Bacetic, J.; Maritzen, T.; Shupliakov, O.; Freund, C.; Haucke, V. 2015: Vesicle uncoating regulated by SH3-SH3 domain-mediated complex formation between endophilin and intersectin at synapses Embo Reports 16(2): 232-239
Milosevic, I.; Giovedi, S.; Lou, X.; Raimondi, A.; Collesi, C.; Shen, H.; Paradise, S.; O'Toole, E.; Ferguson, S.; Cremona, O.; De Camilli, P. 2011: Recruitment of endophilin to clathrin-coated pit necks is required for efficient vesicle uncoating after fission Neuron 72(4): 587-601
Pessano Fialho, M.F.; Thanigai Arasu, G.S.; Chen, S.; Imlach, W.L.; Bunnett, N.W.; Tonello, R. 2025: Endophilin A1 and synaptojanin 1-dependent endocytosis of synaptic vesicles in nociceptive spinal circuits maintains postoperative and cancer pain Pain 2025
Gad, H.; Ringstad, N.; Löw, P.; Kjaerulff, O.; Gustafsson, J.; Wenk, M.; Di Paolo, G.; Nemoto, Y.; Crun, J.; Ellisman, M.H.; De Camilli, P.; Shupliakov, O.; Brodin, L. 2000: Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin Neuron 27(2): 301-312
Candiello, E.; Mishra, R.; Schmidt, B.; Jahn, O.; Schu, P. 2017: Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity Scientific Reports 7(1): 15781
Harris, T.W.; Hartwieg, E.; Horvitz, H.R.; Jorgensen, E.M. 2000: Mutations in synaptojanin disrupt synaptic vesicle recycling Journal of Cell Biology 150(3): 589-600
Wang, Y.; Zhu, Y.; Li, W.; Yan, S.; Li, C.; Ma, K.; Hu, M.; Du, C.; Fu, L.; Sun, J.; Zhang, C.X. 2023: Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1 Journal of Neuroscience: the Official Journal of the Society for Neuroscience 43(36): 6230-6248
Zhang, J.; Tan, M.; Yin, Y.; Ren, B.; Jiang, N.; Guo, G.; Chen, Y. 2015: Distinct Functions of Endophilin Isoforms in Synaptic Vesicle Endocytosis Neural Plasticity 2015: 371496
Sullivan, J.M. 2011: A surprisingly singular role for endophilin in synaptic vesicle recycling Neuron 72(4): 504-505
Sopova, E.; Korenkova, O.; Onochin, K.; Brodin, L.; Shupliakov, O. 2021: P.0557 a role of endophilin a in the synaptic vesicle liquid phase European Neuropsychopharmacology 53: S409-S410
Gad, H.; Shupliakov, O.; Low, P.; Kjaerulff, O.; Ringstad, N.; Decamilli, P.; Brodin, L. 1999: Perturbation of synaptojanin function impairs synaptic vesicle endocytosis at a living synapse Society for Neuroscience Abstracts 25(1-2): 1744
Chen, C.-K.; Bregere, C.; Paluch, J.; Lu, J.F.; Dickman, D.K.; Chang, K.T. 2014: Activity-dependent facilitation of Synaptojanin and synaptic vesicle recycling by the Minibrain kinase Nature Communications 5: 4246
Andersson, F.; Löw, P.; Brodin, L. 2010: Selective perturbation of the BAR domain of endophilin impairs synaptic vesicle endocytosis Synapse 64(7): 556-560
Geng, J.; Wang, L.; Lee, J.Y.; Chen, C.-K.; Chang, K.T. 2016: Phosphorylation of Synaptojanin Differentially Regulates Endocytosis of Functionally Distinct Synaptic Vesicle Pools Journal of Neuroscience: the Official Journal of the Society for Neuroscience 36(34): 8882-8894
Marza, E.; Long, T.; Saiardi, A.; Sumakovic, M.; Eimer, S.; Hall, D.H.; Lesa, G.M. 2008: Polyunsaturated fatty acids influence synaptojanin localization to regulate synaptic vesicle recycling Molecular Biology of the Cell 19(3): 833-842
Kontaxi, C.; Kim, N.; Cousin, M.A. 2023: The phospho-regulated amphiphysin/endophilin interaction is required for synaptic vesicle endocytosis Journal of Neurochemistry 166(2): 248-264
Guichet, A.; Wucherpfennig, T.; Dudu, V.; Etter, S.; Wilsch-Bräuniger, M.; Hellwig, A.; González-Gaitán, M.; Huttner, W.B.; Schmidt, A.A. 2002: Essential role of endophilin A in synaptic vesicle budding at the Drosophila neuromuscular junction EMBO Journal 21(7): 1661-1672
Schmidt, A.; Wolde, M.; Thiele, C.; Fest, W.; Kratzin, H.; Podtelejnikov, A.V.; Witke, W.; Huttner, W.B.; Söling, H.D. 1999: Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid Nature 401(6749): 133-141