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Antibodies to Escherichia coli in young calves: K antigens

Ingram, D.G.; Corbeil, L.B.; Malcomson, M.E.

American Journal of Veterinary Research 31(1): 71-79

1970

ISSN/ISBN: 0002-9645
PMID: 4905594
Accession: 014348708

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Summary
Passive and antiglobulin haemagglutination tests were used, employing four strains of E, coli virulent for cakes as antigens . Almost all of the 38 samples of colostrum had high titres of antibodies against the O antigens. whereas antibody levels were much lower in milk. Serum amples were taken from 30 colostrum-fed and 17 colostrum-deprived calves from birth to five weeks of age. Specific mercaptoethanol-resistant antibodies against the O antigens were present in calf serum within a few hours after colostrum was fed. and attained a level approx. one-quarter of that in the colostrum. Natural production of antibody macroglobulin against the O antigen of strain O101: K(RVC118) commenced as early as two weeks. More colostrum-deprived calves produced antibody against the O antigen. Calves which acquired a high level of antibody from the colostrum did not produce antibody against O antigen during the first five weeks of life. In the same calves, 57-97% of 38 colostrum samples had antibodies to the K antigens of the four strains at fairly high titres. Antibodies were detectable in milk, but occurrence was less and levels were much lower than in colostrum. The anti bodies were not highly specific and were cross-reactive, which may be of importance in increasing the resistance of calves to infection. Again, colostral antibodies were detected in the serum of the calves a few hours after colostrum was fed. Natural pro duction of antibody (macroglobulin) against K antigens was detectable only by the antiglobulin haemagglutination test. Only colostrum-fed calves produced these antibodies during the first five weeks of life.

References

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