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Biochemistry of vaccinia-infected mouse fibroblasts . n. Properties of the chromosomal DNA of infected cells

Kit, Saul; Dubbs, D.R.

Virology 18(2): 286-293

1962

ISSN/ISBN: 0042-6822
Accession: 024240386

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Summary
The properties of L- M fibroblast DNA were investigated following cell infection by vaccinia (IHD). The DNA isolated from cells at 15.5-21 hours post inoculation (PI) was indistinguishable from DNA of control cells with respect to the molecular extinction coefficient and the banding in CsCl density gradients. The intrinsic viscosity was only slightly lowered. DNA isolated from autolyzed cells 2 days after vaccinia addition was considerably degraded. The effect of vaccinia upon the DNA of cells prelabeled with thymidine- H3 was studied. The total DNA radioactivity of infected cells declined by less than 10% in 24 hours, and there was no accumulation of radioactivity in the acid-soluble pool. No radioactivity was detected in partially purified vaccinia harvested 14.5 hours PI from prelabeled cells. The effect of bromodeoxyurldine (BUDr) on propagation of vaccinia in normal L-M cells and in L-M cells resistant to BUDr [L-M (BU-25)] has been investigated. Although L-M (BU-25) and L-M cells appear to be equally susceptible to infection with vaccinia and undergo similar pathological changes, the infectious virus yield from both cell lines in medium containing BUDr was about 2% of that in normal medium.

References

Kit, S.; Dubbs, D.R. 1962: Biochemistry of vaccinia-infected mouse fibroblasts (strain L-M). II. Properties of the chromosomal DNA of infected cells Virology 18: 286-293
Kit, S.; Dubbs, D.R. 1963: Biochemistry of vaccinia-infected mouse fibroblasts . IV. Thymidine-3H uptake into DNA of cells exposed to cold shock Exptl Cell Res 31(2): 397-406
Kit, S.; Dubbs, D.R. 1963: Biochemistry of Vaccinia-Infected Mouse Fibroblasts (Strain L-M). Iv. 3h-Thymidine Uptake into Dna of Cells Exposed to Cold Shock Experimental cell research 31: 397-406
Kit, Saul; Dubbs, D.R.; Hsu, T.C. 1963: Biochemistry of vaccinia-infected mouse fibroblasts . III. Radioautographic and biochemical studies of thymidine-H3 uptake into DNA of L-M cells and rabbit cells in primary culture Virology 19(1): 13-22
Kit, S.; Dubbs, D.R.; Hsu, T.C. 1963: Biochemistry of vaccinia-infected mouse fibroblasts (strain L-M). III. Radioautographic and biochemical studies of thymidine-H3 uptake into DNA of L-M cells and rabbit cells in primary culture Virology 19: 13-22
Kit, S.; Dubbs, D.R. 1962: Biochemistry of vaccinia-infected mouse fibroblasts (strain L-M) Virology 18(2): 274-285
Blawat, F.; Zawistowski, S.; Kowalska, Z. 1964: Cytochemical Studies of Vaccinia-Virus-Infected Cells. Ii. Activity of some Enzymes in Vaccinia-Virus-Infected Cultures of Mouse L Fibroblasts, Clone 929 Biuletyn Instytutu Medycyny Morskiej w Gdansku 15: 123-129
Kit, Saul; Dubbs, D.R. 1962: Biochemistry of vaccinia-infected mouse fibroblasts . I. Effects on nucleic acid and protein synthesis Virology 18(2): 274-285
Kit, S.; Dubbs, D.R. 1962: Biochemistry of vaccinia-infected mouse fibroblasts (strain L-M). I. Effects on nucleic acid and protein synthesis Virology 18: 274-285
Domber, E.; Holowczak, J.A. 1986: Vaccinia virus proteins on the plasma membranes of infected cells. IV. Studies employing L cells infected with ultraviolet-irradiated vaccinia virions Virology 152(2): 331-342
Kit, S.; Piekarski, L.J.; Dubbs, D.R. 1963: Induction of thvmidine kinase by vaccinia-infected mouse fibroblasts Jour Molecular Biol 6(1): 22-33
Kit, S.; Piekarski, L.J.; Dubbs, D.R. 1963: Induction of thymidine kinase by vaccinia-infected mouse fibroblasts Journal of Molecular Biology 6: 22-33
Kit, S.; Piekarski, L.J.; Dubbs, D.R. 1963: Induction of thymidine kinase by vaccinia-infected mouse fibroblasts Journal of Molecular Biology 6(1): 22-33
Mallon, V.R.; Domber, E.A.; Holowczak, J.A. 1985: Vaccinia virus proteins on the plasma membranes of infected cells. II. Expression of viral antigens and killing of infected cells by vaccinia virus-specific cytotoxic T cells Virology 145(1): 1-23
Barbeyron, T.; Mars, M.; Schroeder, E.; Malpièce, Y.; Plucienniczak, A.; Beaud, G.; Streeck, R.E. 1987: Identification of vaccinia promoters by heterologous expression of hepatitis B surface antigen in mouse cells infected by recombinant vaccinia viruses Biochimica et Biophysica Acta 910(3): 240-244
Nishimura, C. 1965: Properties of Nucleic Acids in a Purified Vaccinia Virus and in Vaccinia-Infected Hela Cells Japanese Journal of Medical Science and Biology 18: 121-126
Blawat, F.; Zawistowski, S.; Kowalska, Z. 1964: Cytochemical Studies of Vaccinia-Virus-Infected Cells. I. Activity of some Enzymes in Vaccinia-Virus-Infected Fl Cells of the Human Amnion Biuletyn Instytutu Medycyny Morskiej w Gdansku 15: 5-13
Blawat, F.; Zawistowski, S.; Kowalska, Z.; Towiańska, A. 1965: Cytochemical studies of vaccinia-virus-infected cells. IV. the behaviour of some hydrolytic enzymes in vaccinia-virus-infected cultures of FL and L cells Biuletyn Instytutu Medycyny Morskiej W Gdansku 16(3): 139-146
Kit, S.; Dubbs, D.R.; Piekarski, L.J. 1963: Inhibitory effects of p-fluoro-phenylalanine and puromycin on the induction of thymidine kinase by vaccinia-infected mouse fibroblasts Science 138(3544): 992
Blawat, F.; Zawistowski, S.; Kowalska, Z.; Towianska, H. 1965: Cytochemical Studies of Vaccinia-Virus-Infected Cells. 3. Further Studies on the Activity of Oxidative Enzymes in Vaccinia-Virus-Infected Cultures of Fl and L Cells Biuletyn Instytutu Medycyny Morskiej w Gdansku 16: 7-20