FOn the demonstration of the action of typhus vaccine and sera through blood cultures in experiments on mice

Lipp, R.; Lipp, R

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FOn the demonstration of the action of typhus vaccine and sera through blood cultures in experiments on mice

Lipp, R.

Zeitschr Immunitatsforsch 114(2/3): 235-243

1957

Accession: 024673716

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Summary

A test was described in which the presence or absence of a bacteremia served as a criterion of effectiveness of sera and vaccines. One-, 4- and 24-hours after infection of mice with typhus Ty 2, a drop of tail blood was incubated for 48 hours at 37° C, and tested with 9-Vi-factor serum. Five to 10 million cells are required for production of bacteremia in 100% of mice; 20 million for the LD50. Differences between methods of produc- tion of both active and passive immunity could be detected. Peritonitis, embolism, and intoxications do not complicate the test because of the small dose and early sampling. The method will be developed further.