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Role of JNK signal transduction pathway on apoptosis of human kidney tubular epithelial cells induced by ochratoxin A in vitro

Li-Zeng-Ning; Xing-Ling-Xiao; Cui-Jin-Feng; Shen-Hai-Tao; Ding-Tao; Yan-Xia; Wang-Jun-Ling; Zhang-Xiang-Hong

Zhongguo Yaolixue Yu Dulixue Zazhi 22(4): 291-295

2008

Accession: 033258653

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Summary
AIM To explore the role of e-Jun NH, terminal kinase (JNK) singnal transduction pathway on ochratoxin A (OA) inducing apopLosis of human kidney tubular epithelial cells HKC) in. vilro. METHODS HKC were incubated with saline, solvent (0. 04% ethanol), 1 mu mol.L-1 OA and JNK inhibitor SP600125 (0.5 mu mol.L-1') + OA, respectively, for 24 h. The apoptosis rate, the expression of caspase 3 and level of p-JNK of HKC were detected by flow cytometry, immunocytochemical staining and Western blot, respectively. RESULTS After OA treatment, the apoptosis rate was higher than that in solvent group [(4.24 +/- 0.17) % vs (1.06 +/- 0.14) % ]. Pretreatment with SP600.125 for 30 min decreased the apoplosis rate [(2.44 +/- 0.38)]. The expressions of caspase 3 and level of p-JNK in OA group were higher than that in solvent group, while both were lower in SP600125 + OA group than that in OA group. CONCLUSION The possible mechanism of apoptosis of HKC -after OA treatment may be related with the activation of JNK and increasing the expression of caspase 3.

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