Ca2+ transport in erythrocytes from patients with Duchenne muscular dystrophy

Pijst, H.L.; Scholte, H.R.; Pijst, H.L; Scholte, H.R

doi:10.1016/0022-510x(83)90152-1

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Ca2+ transport in erythrocytes from patients with Duchenne muscular dystrophy

Pijst, H.L.; Scholte, H.R.

Journal of the Neurological Sciences 60(3): 411-417

1983

ISSN/ISBN: 0022-510X

PMID: 6138395

DOI: 10.1016/0022-510x(83)90152-1
Accession: 042430655

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Summary

Erythrocyte membranes, isolated after step-wise hemolysis in buffered sucrose (Tris-sucrose membranes), showed higher Ca2+ Mg2+-ATPase activity than erythrocyte membranes obtained after hemolysis in Tris-HCl, and washing in EDTA (Tris-EDTA membranes). In Tris-sucrose membranes, the activity was not stimulated by the addition of monovalent cations, or calmodulin. Differences reported for the activity and properties of Ca2+ Mg2+-ATPase between Tris-EDTA membranes of Duchenne patients and controls, were not found in Tris-sucrose membranes. Also the activity and properties of the Ca2+-pump in resealed erythrocyte membranes were not changed.