Release of norepinephrine-3H and serotonin-3H evoked from brain slices by electrical-field stimulation-calcium dependency and the effects of lithium, ouabain and tetrodotoxin

Katz, R.I.; Kopin, I.J.; Katz, R.I; Kopin, I.J

doi:10.1016/0006-2952(69)90289-5

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Release of norepinephrine-3H and serotonin-3H evoked from brain slices by electrical-field stimulation-calcium dependency and the effects of lithium, ouabain and tetrodotoxin

Katz, R.I.; Kopin, I.J.

Biochemical Pharmacology 18(8): 1835-1839

1969

ISSN/ISBN: 0006-2952

PMID: 5811621

DOI: 10.1016/0006-2952(69)90289-5
Accession: 044190591

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Summary

Slices of mammalian brain accumulate tritiated norepinephrine or serotonin when incubated in a medium with these compounds. Mild electrical stimulation of short duration induces a striking increase in the release of exogenous-labeled amine. Electrically stimulated release of norepinephrine-3H, but not of serotonin-3H, is calcium dependent. Stimulation-induced release of both monoamines is significantly diminished by the addition of ouabain, lithium or tetrodotoxin to the perfusing medium. Elevated calcium concentration prevents lithium-induced inhibition of norepinephrine-3H release but has no effect on lithium inhibition of evoked release of serotonin-3H. Enhanced calcium levels did not reverse ouabain-induced inhibition of release of either monoamine.