Recombinase Polymerase Amplification and its Applications in Parasite Detection

Zheng, W.-b.; Wu, Y.-d.; Ma, J.-g.; Zhu, X.-q.; Zhou, D.-h.; Zheng, W.-b; Wu, Y.-d; Ma, J.-g; Zhu, X.-q; Zhou, D.-h

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Recombinase Polymerase Amplification and its Applications in Parasite Detection

Zheng, W.-b.; Wu, Y.-d.; Ma, J.-g.; Zhu, X.-q.; Zhou, D.-h.

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 33(5): 382-386

2015

ISSN/ISBN: 1000-7423

PMID: 26931046

Accession: 058708994

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Summary

Recombinase polymerase amplification (RPA) is a recently -developed isothermal nucleic-acid-amplification technology that is based on the nucleic acid replication mechanism in T4 bacteriophage. With this technique, nucleic-acid templates can be amplified to measurable levels within 20 min at 37-42 °C. The. RPA process has high sensitivity and specificity, and is simple to operate, thus nucleic acids can be detected rapidly in non-laboratory conditions. Since its development in 2006, the RPA technique has been applied in agriculture, food safety, medicine, transgene detection, etc. In this review, we will give an overview on the research progress of RPA and its application in parasite detection.