Immune repertoire analysis of normal Chinese donors at different ages

Song, C.; Pan, W.; Brown, B.; Tang, C.; Huang, Y.; Chen, H.; Peng, N.; Wang, Z.; Weber, D.; Byrne-Steele, M.; Wu, H.; Liu, H.; Deng, Y.; He, N.; Li, S.; Song, C; Pan, W; Brown, B; Tang, C; Huang, Y; Chen, H; Peng, N; Wang, Z; Weber, D; Byrne-Steele, M; Wu, H; Liu, H; Deng, Y; He, N; Li, S

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Immune repertoire analysis of normal Chinese donors at different ages

Song, C.; Pan, W.; Brown, B.; Tang, C.; Huang, Y.; Chen, H.; Peng, N.; Wang, Z.; Weber, D.; Byrne-Steele, M.; Wu, H.; Liu, H.; Deng, Y.; He, N.; Li, S.

Cell Proliferation 55(11): E13311

2022

ISSN/ISBN: 1365-2184

PMID: 35929064

Accession: 080399703

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Summary

This study investigated the characteristics of the immune repertoire in normal Chinese individuals of different ages. In this study, all seven receptor chains from both B and T cells in peripheral blood of 16 normal Chinese individuals from two age groups were analyzed using high-throughput sequencing and dimer-avoided multiplex PCR amplification. Normal in this study is defined as no chronic, infectious or autoimmune disease within 6 months prior to blood draw. We found that compared with the younger group, the clonal expression of T-cell receptor repertoire increased in the older group, while diversity decreased. In addition, we found that the T-cell receptor repertoire was more significantly affected by age than the B-cell receptor repertoire, including significant differences in the use of the unique TCR-alpha and TCR-beta V-J gene combinations, in the two groups of normal participants. We further analyzed the degree of complementarity determining region 3 sequence sharing between the two groups, and found shared TCR-alpha, TCR-gamma, immunoglobulin-kappa and immunoglobulin-lambda chain complementarity determining region 3 sequences in all subjects. Taken together, our study gives us a better understanding of the immune repertoire of different normal Chinese people, and these results can be applied to the treatment of age-related diseases. Immune repertoire analysis also allows us to observe participant's wellness, aiding in early-stage diagnosis.

Immune repertoire analysis of normal Chinese donors at different ages

Song, C.; Pan, W.; Brown, B.; Tang, C.; Huang, Y.; Chen, H.; Peng, N.; Wang, Z.; Weber, D.; Byrne-Steele, M.; Wu, H.; Liu, H.; Deng, Y.; He, N.; Li, S.

Cell Proliferation 55(11): E13311

2022

Full-Text Article emailed within 1 workday

Summary

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