Investigating the effects of biodegradable microplastics and copper ions on probiotic (Bacillus amyloliquefaciens): Toxicity and application

Li, R.; Tao, J.; Huang, D.; Zhou, W.; Gao, L.; Wang, X.; Chen, H.; Huang, H.; Li, R; Tao, J; Huang, D; Zhou, W; Gao, L; Wang, X; Chen, H; Huang, H

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Investigating the effects of biodegradable microplastics and copper ions on probiotic (Bacillus amyloliquefaciens) : Toxicity and application

Li, R.; Tao, J.; Huang, D.; Zhou, W.; Gao, L.; Wang, X.; Chen, H.; Huang, H.

Journal of Hazardous Materials 443(Part A): 130081

2023

ISSN/ISBN: 1873-3336

PMID: 36367472

Accession: 080653478

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Summary

Currently, microplastic pollution is more serious and complicates the toxic effects of other co-existing pollutants in the environment. However, the effect and mechanism of biodegradable plastics on the growth and metabolism of probiotic remain unclear. This work selected Bacillus amyloliquefaciens as model bacterium for a three-day exposure experiment to probe the issues. The results showed that 100 mg/L polylactic acid microplastics (PLA MPs) (3-4 mm, flake shape) caused oxidative damage to cell membranes, disrupted cell wall composition and inhibited cell growth by 21.2-27.5 %. The toxicity was not simply additive or synergistic effects when PLA MPs (100 mg/L) and copper ions (10 mg/L) coexisted. PLA MPs did not significantly increase the toxicity of copper to bacteria, instead triggered some mechanisms to resist the toxicity of copper. The bacteria formed spores to resist PLA MPs, while the copper ions toxicity was weaken by chelation and efflux. It is worth noting that copper ions instead increased the expression of genes related fengycin and iturin then improving the bacteriostatic activity of the probiotic. This paper deeply analyzes the toxicity mechanism of combined pollution on Bacillus amyloliquefacien, and also provides new perspective for helping to inhibit pathogenic bacteria under biodegradable microplastics and metal stress.