Top Buy Now Summary References
Home
>
Section
>
Chapter

A Comparative Study between Plant and Callus Extracts of Abutilon indicum (L.) Sweet: Antioxidant, Antibacterial, Antidiabetic and Anti-Proliferative Activity

Sireen, A.A.; Anbumalarmathi, J.

International Journal of Biochemistry Research-Review: 13-24

2020

ISSN/ISBN: 2231-086X
Accession: 080970978

Full-Text Article emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Summary
Abutilon indicum is consider to be used in the traditional system of medicine. It is found in tropical and subtropical regions of the world. It is used to treat various diseases. This plant does not cause any side effects to humans. As the plant has wide variety of medicinal properties, the present study aimed to comparative between plant and callus extract of Abutilon indicum (L.) sweet for antioxidant, antibacterial, antidiabetic and anti- proliferative activity. The highest percentage of callus induction (89.50%) and callus weight (1.26 g) was observed in T5 (MS + 2, 4-D (2.5 mg/l) + BAP (2 mg/l) and T8 [IBA (4 mg/l)] respectively. Phytochemical analysis of aqueous and ethyl acetate extracts of A. indicum in vivo plant and in vitro grown callus showed the presence of alkaloids, flavonoids, phenols, carbohydrates, glycosides, protein, terpenoids, saponins, tannins and coumarin. The total phenolic content was high in aqueous extract of callus (30.68 mg TAE/g). Maximum DPPH radical scavenging activity was found in aqueous extract of callus (86%) with IC50 value of 68.49 µg/ml. FT-IR analysis of aqueous extract of A. indicum plant and callus showed the presence of characteristic stretching at 2930.28 and 2927.75 indicating the presence of C-H stretching respectively. GC-MS analysis revealed the presence of 17 compounds in ethyl acetate plant extract, whereas 7 compounds in ethyl acetate callus extract such as tetradecane, 1-chloro, Sulfurous acid 2-prophytridecyl ester and 1- ethyl-3-[2-(octadecylthio) ethyl] thiourea. The ethyl acetate extracts of callus and plant and was found to be effective against Bacillus subtilis (3.1 mm) and Staphylococcus aureus (2.9 mm). Maximum α-amylase inhibitory activity was observed in aqueous callus extract (32.65%) with IC50 value of 833.61 µg/ml. HeLa cell viability was found to be 26.8% and 21.8% in plant and callus extract respectively.

References

Yasmin, S.; Kashmiri, M.A.; Asghar, M.N.; Ahmad, M.; Mohy-ud-Din, A. 2010: Antioxidant potential and radical scavenging effects of various extracts from Abutilon indicum and Abutilon muticum Pharmaceutical Biology 48(3): 282-289
Rojsanga, P.; Bunsupa, S.; Brantner, A.H.; Sithisarn, P. 2017: Comparative Phytochemical Profiling and In Vitro Antioxidant Activity of Extracts from Raw Materials, Tissue-Cultured Plants, and Callus of Oroxylum indicum (L.) Vent Evidence-Based Complementary and Alternative Medicine: Ecam 2017: 6853212
Chandrashekhar, V.M.; Nagappa, A.N.; Channesh, T.S.; Habbu, P.V.; Rao, K.P. 2004: Anti-diarrhoeal activity of Abutilon indicum Linn leaf extracts Journal of Natural Remedies 4(1): 12-16
Krisanapun, C.; Lee, S-Ho.; Peungvicha, P.; Temsiririrkkul, R.; Baek, S.Joon. 2011: Antidiabetic Activities of Abutilon indicum (L.) Sweet Are Mediated by Enhancement of Adipocyte Differentiation and Activation of the GLUT1 Promoter Evidence-BasedComplementaryandAlternativeMedicine:Ecam 2011: 167684
Merritta, S.; Sandra, J.; Jisha, S.; Sivaprasad, A.N. 2026: Comparative Analysis of Phytochemical Constituents and Antibacterial Activity of Callus and Whole Plant Extracts of Aristolochia indica L. Research Journal of Biotechnology 21(5): 39-46
Tauchen, J.; Doskocil, I.; Caffi, C.; Lulekal, E.; Marsik, P.; Havlik, J.; Van Damme, P.; Kokoska, L. 2015: In vitro antioxidant and anti-proliferative activity of Ethiopian medicinal plant extracts Industrial Crops and Products 74: 671-679
Seth, S.; Panigrahi, J. 2019: In vitro organogenesis of Abutilon indicum (L.) Sweet from leaf derived callus and assessment of genetic fidelity using ISSR markers Journal of Horticultural Science and Biotechnology 94(1): 70-79
Mata, R.; Nakkala, J.Reddy.; Sadras, S.Rani. 2015: Biogenic silver nanoparticles from Abutilon indicum: their antioxidant, antibacterial and cytotoxic effects in vitro Colloids and Surfaces. B Biointerfaces 128: 276-286
Tian, C.; Zhang, P.; Yang, C.; Gao, X.; Wang, H.; Guo, Y.; Liu, M. 2018: Extraction Process, Component Analysis, and in Vitro Antioxidant, Antibacterial, and Anti-Inflammatory Activities of Total Flavonoid Extracts from Abutilon theophrasti Medic. Leaves Mediators of Inflammation 2018: 3508506
Nevinnykh, V.A. 1936: On the hybrids between the species Abutilon avicennae Gaertn. and Abutilon indicum Sweet Priroda (Nature), 10, 99-103
Sharma,S.K.; SumitraSingh; NaveenGoyal 2008: Anti-inflammatory effects of various extracts of root of Abutilon indicum Linn International Journal Of Chemical Sciences: 3, 1236-1240
Poonkothai, M. 2006: ANTIBACTERIAL ACTIVITY OF LEAF EXTRACT OF Abutilon indicum Ancient Science of Life 26(1-2): 39-41
Ranganath, T.; Rahamat, K.; Anbalagan, S.; Muruganantham, S. 2026: Phytochemical Profiling and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities of Abutilon indicum L. Ethanolic Leaf Extracts Research Journal of Biotechnology 21(1): 190-201
El Bakry, A A.; Mostafa, H A M.; Alam, E. A. 2014: Antioxidant and antibacterial activity of callus and adventitious root extracts from Rumex vesicarius L Journal of Medicinal Plants Research 8(12): 479-488
Seetharam, Y.N.; Chalageri, G.; Setty, S.Ramachandra.; Bheemachar 2002: Hypoglycemic activity of Abutilon indicum leaf extracts in rats Fitoterapia 73(2): 156-159
Ernawita; Wahyuono, R.A.; Hesse, J.; Hipler, U.-C.; Elsner, P.; Böhm, V. 2017: In Vitro Lipophilic Antioxidant Capacity, Antidiabetic and Antibacterial Activity of Citrus Fruits Extracts from Aceh, Indonesia Antioxidants 6(1)
Al-Hadid, K.J.; Al-Karablieh, N.; Abu-Irmaileh, B.; Sharab, A.; Al Jaafreh, A.M. 2022: Antibacterial, Antifungal, Antioxidant, and Anti-Proliferative Effects of Eucalyptus camaldulensis and Pistacia atlantica Ethanol Extracts Tropical Journal of Natural Product Research 6(2): 207-212
Venkateswarlu, K.; Vijayabhaskar, K.; Krishna, O. S.; Devanna, N.; Sekhar, K. B. C. 2015: Evaluation of Anti-ulcer Activity of Hydro Alcoholic Extracts of Abutilon indicum, Helianthus annuus and Combination of Both against Ethanol and Pyloric Ligation Induced Gastric Ulcer in Albino Wistar Rats British Journal of Pharmaceutical Research 5(1): 42-51
Banerjee, S.; Phuneerub, P.; Jaidee, W.; Rujanapun, N.; Duangyod, T.; Malee, K.; Maneerat, W.; Suthiphasilp, V.; Laphookhieo, S.; Ramli, S.; Mah, S.H.; Chansukh, K.K.; Hiransai, P.; Puttarak, P.; Sarker, S.D.; Nahar, L.; Charoensup, R. 2025: Abutilon indicum (L.) Sweet Extracts Inhibit Key Glucose Metabolic Enzymes While Enhancing Glucose Transport in L6 Myotubes and 3T3L1 Adipocytes Journal of Food Biochemistry 2025(1): 8252812
SammiaYasmin; Kashmiri,M.A.; IftikharAhmad; AhmadAdnan; MushtaqAhmad 2008: Biological activity of extracts in relationship to structure of pure isolates of Abutilon indicum Pharmaceutical Biology: 10-11, 673-676