Effect of protease inhibitors on autolysis of carp muscle homogenate

Makinodan, Y.; Toyohara, H.; Ikeda, S.; Makinodan, Y; Toyohara, H; Ikeda, S

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Effect of protease inhibitors on autolysis of carp muscle homogenate

Makinodan, Y.; Toyohara, H.; Ikeda, S.

Nippon Suisan Gakkaishi 49(3): 481-484

1983

ISSN/ISBN: 0021-5392

Accession: 005265074

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Summary

To assist the search of proteinases which participate in fish muscle autolysis, the effect of protease inhibitors on the autolysis was investigated. The autolysis was done in the presence of 3 mM N3Na at 37.degree. C. The degree was determined by the colorimetric quantitative assay of 5% trichloroacetic acid soluble fraction after the autolysis and by the qualitative method of comparing electrophoretic patterns. Muscle homogenate was used instead of muscle block from the necessity of mixing inhibitor. Though the autolysis of homogenate proceeded faster than that of block, it was considered to reflect roughly the autolysis of muscle block. The effects of inhibitors were similar in the quantitative and the qualitative methods. Soybean trypsin inhibitor, PCMB and pepstatin had no effect, but EDTA tended to inhibit. The participation of metallo proteinase in the autolysis was indicated. But CaCl2 had no effect. The effect of leupeptin suggested the participation of serine or cysteine proteinase. Though iodoacetic acid showed a remarkable effect, it is still not clear whether the effect depends on proteinase.

Effect of protease inhibitors on autolysis of carp muscle homogenate

Makinodan, Y.; Toyohara, H.; Ikeda, S.

Nippon Suisan Gakkaishi 49(3): 481-484

1983

Full-Text Article emailed within 1 workday

Summary

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