Effect of protein synthesis inhibitors on xylose uptake and iodine 125 insulin binding by rat soleus muscle

Y.K.T.; Gould, M.K.; Y.K.T; Gould, M.K

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Effect of protein synthesis inhibitors on xylose uptake and iodine 125 insulin binding by rat soleus muscle

Y.K.T.; Gould, M.K.

Biochemical and Biophysical Research Communications 87(1): 9-16

1979

ISSN/ISBN: 0006-291X

Accession: 005265288

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Summary

Prolonged exposure (90-180 min) to cycloheximide (0.2 mg/ml), puromycin (0.2 mg/ml) or chloramphenicol (0.1 mg/ml) did not effect 125I-insulin binding by rat soleus muscle. Chloramphenicol (2 mg/ml) depressed insulin binding and insulin-stimulated xylose uptake; these effects were atrributed to the toxic effect of chloramphenicol on muscle ATP levels. Cycloheximide and puromycin inhibited insulin-stimulated xylose uptake without affecting ATP. Puromycin and chloramphenicol, but not cycloheximide, also inhibited basal sugar transport. This difference, and the rapid onset of all these inhibitory effects, suggest that they are not due to the inhibition of protein synthesis, but rather to some more direct effect on sugar transport itself.