Improved method for the isolation of purified mouse peritoneal macrophages
Schon-Hegrad, M.A.; Holt, P.G.
Journal of Immunological Methods 43(2): 169-173
1981
ISSN/ISBN: 0022-1759 PMID: 7264322 DOI: 10.1016/0022-1759(81)90020-x
Accession: 005641911
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Summary
Mouse peritoneal macrophages were cultured for 45 min in medium supplemented with fetal calf serum (FCS) in petri dishes coated overnight with heat-inactivated FCS. After nonadherent cell removal by washing, adherent cells were detached by a brief incubation in the presence of sub-toxic EDTA levels. Overall peritoneal macrophage recoveries of 90% can be routinely achieved with this method, and full cell viability is maintained.
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